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Micro Quiz One
Terms in this set (49)
(T/F) Growth media can consist of an endless variety of ingredients but there are no essentials.
In order for bacteria to grow, it requires a carbon source and nitrogen source to allow bacterial growth.
A defined medium is one which _____ of the components are known.
A complex medium is when one or two ingredients are _________.
_________ are nutrient rich that are from milk, yeast and beef and are broken down by a collection of _________. They are filled with ________, _________ and ________ which allows the growth for a variety of organisms.
A selective medium is one that __________ the growth of a group of organisms.
_________ has bile salts and _______ violet dye that binds to the gram ______ and breaks their cell wall.
An enriched medium is one that contains ingredients that __________ the _______ of organisms. If you do not have these specific components, the compound will not grow.
A differential medium is one that allows you to _________ _____ if they are different.
Does it prevent or promote? and what are some examples
No, it does neither.
pH, fermentation of lactose
What is the main object of streaking?
You want to isolate a single organism and making sure that it is pure.
What is a pure culture?
After growing it on the plate, you look at the plate and see if all of the colonies are the same. Each colony arises from a single cell.
If you were to cross streaks by mixing loop one and four, what would happen?
The purpose of it is to get a single colony, the 1st streak is know to have the most clumped cells, it would erase all of the dilution process and you would have fewer single colonies. More bigger colonies.
What is the purpose gram stain experiment?
To differentiate if it is a gram positive or a gram negative ONLY IN prokaryotes.
Gram positive cells have ________ _____________ _________ and a small ___________ _________. It contains two sugars, N-______________ and N-_______________ acid, which are linked to amino acids.
thick peptidoglycan walls
Gram negative cells have a ________ ___________ space and a lipid outer membrane that is made of _____________ which surrounds the thin peptidoglycan layer
The steps of gram staining
_______ _______ is poured on the and stains both the types are ___________. Next, _________ is placed on the cells and this creates a complex combining with the dye. The cells are still _________ but they are bigger. Next, ___________ is added and this __________ the pores of the _____________ wall. Since, gram _____ does not have one, the complex is able to be washed away. But, the gram ______ cannot be removed meaning it stays there. Lastly, __________-O is added and this stains both the cells ________ but the _________ masks the color.
Sources of error with gram staining
Too many cells on a slide can lead to false gram positive; it gets stuck
Use fresh culture
Heat fixing might break the cells wall/membrane
EMB media contains ________ and _________ dyes that are taken up by _______ ____________ organisms that grow on the plate making them dark colored. ___________ is the main _________ source of this media but sucrose is also present. This also prevents the growth of gram ___, making this _________; When a gram ___ bacteria ferments lactose in this medium, the dark colored colonies produce a _______ or _______ metallic. Just because you see this does not mean it is pure.
Mannitol Salt agar contains high concentrations of _________ which _______ the growth of many microbe that cannot tolerate. This dye also contains a _____ indicator to see if it can ferment ________ to _______, if it does the ph is __________. The indication of red means it is ________ and yellow means it is _________ (fermentation). This dye is complex, ___________ and __________
S1 Agar plate contains ________ _______ __________ (SLS). This is a detergent which alters the ______ _______ of gram ___ and prevents the growth. It also contain the antibiotic _____________ which is most active against ________ anaerobic gram ___ bacteria. This works by inhibiting the enzyme __________ ____________ which blocks _____________ ______ and synthesis. _________ and __________ also produces an osmotic stress that some organisms cannot tolerate.
sodium louroyl sarcosine
The goal of the of this S1 agar is to isolate an organism that produces a ______________ pigment which is shown by a ________ change in the media. This is caused by the secretion by the ___________ of the bacterial cell. and then ___________ into the medium. This is a _________-soluble pigment. The yellow green pigment is called __________ and this is an _______ ___________. The bacteria moves into the medium and then scavenges on the ___________ to give to the cell.
Endospores are capable of __________ unfavorable growth conditions. A spore forming bacterium produces a single __________ once the environment is not suitable for the organism. The ___________ cell dies and leaving the ________ behind. These spores have _________ dense layers which protect the ______. Once the conditions are favorable, the spore will ___________ to produce a ____________ cell.
The ____________ agar will not be separating spore formers vs non-sporeformers, rather __________ the sample will kill the ___________ cells and allow the nutrient agar to give the __________ to the cells.
Sodium lactate plates contain _________ as the main _______ source which not all organisms are capable of utilizing.
requires oxygen in order for it to grow
Uses oxygen, but without it can grow as well
Cannot use oxygen but the presence will not harm the organism
Cannot grow with oxygen and the presence of it will slow down the growth
_____ phase cells are growing at the __________ rate and at a _______ interval. The cells are ________ and are metabolically _________. As the cells divide, the __________ are being spread out and become ________, then the _______ products get larger. This then causes the it to enter the ___________ phase,
What is the purpose of the catalase test?
to identify if the unknown has the enzyme catalase
The role of the electron transport chain
To harvest energy from the electrons in order to generate a protein gradient. This allows the system to create ATP.
One molecule in the ETC, __________, can transfer electrons directly to __________ and creating oxygen ____________. For example, (H202- and 02-), and these are ________ _______ and can damage ______, _______ and other important molecules in the cell.
To prevent damage by these radicals, _________ utilizing organisms posses _________ to break down the compounds. These are called ___________ __________ and __________
Superoxide dismutase reaction
2H+ + 2O2- --> H2O2+O2
2H2O2 --> 2H20 + O2
We are looking for the enzyme catalase so we do this by adding _________ to the cells, if it generates _________, it shows the breakdown of the compound and the formation of ________ ______.
What is the purpose of the oxidase test?
To determine if the organism possesses the cytochrome C oxidase
in the ETC, there are several types of ___________ complexes that do the passage of ________ to oxygen. Not all bacteria possess the same type so we want to specifically see if this one has it.
The oxidase paper we are using contains the dye, N-N- __________-_______________. If cytochrome C is present, it will be able to _________ this dye and cause a color change to a ______ or ___________- ________ color.
Why use a wooden stick rather the innoculating loop?
The iron in the loop might get onto the paper and have the ability to oxidize the N-N-dimethylphenylenediamine
What is the purpose of the phenol red test?
To determine if the organism can ferment glucose into acid
What is fermentation?
This is a energy generating process that occurs without oxygen. It can create acid, carbon dioxide also
What does the amogel do?
You place that into your durham tube to create an anaerobic environment.
If glucose is fermented into acid, this will ________ the pH, which then changes the color from ______ to ________. If there is gas produced, the amogel will be __________ ____. In the absence of your ___________ source, ex __________, the organism cannot produce _______ and the media will stay _____.
What is the purpose of the minimal media test?
To determine if the organism needs vitamins and sugars in order to grow
Minimal media contains __________ __________ as the carbon source and _____________ _________ as the nitrogen source. If the media is _________, the isolate does not need _________ or ___________ in order for growth this means that it is _________.
What is the purpose of the fluorescent pigment test?
To determine if your organism produces a water soluble fluorescent pigment
King's B media contains __________ and _________ which increases the production of ______________. But the difference is that it lacks the compounds to _______ growth.
Little or no growth with oxygen
Lots of growth in anaerobic
Lots of growth with oxygen
little growth in anerobic
Same amount of growth for both
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