Transport of Oxygen and Carbon Dioxide in Body Fluids
Folder C #9
review: how do temp, pressure, and salt affect gas solubility?
increase solubility by: decreasing temp increasing pressure decreasing salt
Partial pressure of oxygen (sea level, full humidity)
atmospheric: 152 mmHg alveolar: 104mmHg (lower b/c mix of old and new air) Venous: 40mmHg (on way back to lungs, nice [ ] gradient: 104:40
oxygen levels in plasma
0.3 ml O2/100 ml plasma
oxygen levels in blood (whole blood)
20 ml O2/100 ml plasma...
difference from 0.3 due to hemoglobin! 60x as much! can have larger metabolism with hemoglobin
low levels of Red Blood Cells (RBCs) reduced ability to transport oxygen
similar across species heme group...in the middle sits IRON...which can bind oxygen 2 Beta globins, 2 alpha globins
hemoglobin: fetal forms
slightly different protein fetal heme group has a HIGHER affinity to oxygen than adult we want oxygen from mom's blood to diffuse to baby's blood... preferentially moving O2 from mom to baby
expect to see this in chickens?
nope! no placenta...develop in egg instead. over time, affinity decreases
why is the affinity between hemoglobin and O2 so important?
pick up and drop off
oxygen equilibrium curve axis
partial pressure of oxygen in the blood (x axis) hemoglobin percent saturation (y axis)
what's the shape of the graph?
SIGMOID!...get the curves right...
oxygen binding to heme groups...
once one binds, the subsequent oxygens have an easier time binding due to the conformational change in the heme group...makes the curve rise faster with each subsequent oxygen added
RAPID loading and unloading of oxygen due to...
conformational shape induced by binding of oxygen to heme...
steep part of the curve
small change in [O2] has an enormous effect on % saturation see the steep part in the lungs and tissues... QUICK pickup and dropoff of O2
plateau of curve
can't saturate anymore... almost all of hemes are occupied even a huge change of partial pressure of O2, we barely affect % saturation safety margin of error...see next notecard
safety margin of error:
we can still maintain oxygen delivery to tissue ex: altitude (pressure goes down...concentration the same but particles more spread out...larger volume), mild altitude, blood doesn't notice
hookah (oxygen) bar...
gimmick! can't add anymore O2 to lungs/body but will deplete CO2 concentrations...very dangerous for regulation (CO2 triggers breathing cycle and important for buffer system)
inflammation in lungs due to infection/fluid in lungs... slower diffusion rates because molecules have larger distance to cross
damage to lungs due to alveolar collapse...more CO2 means less room for O2
general roles of plateau and steep part of curve
plateau: protective... steep part: allows for quick diffusion...essential
in muscle...one subunit of hemoglobin allows some muscle fibers to store O2 myoglobin curve NOT sigmoid... at low partial pressures of O2, myoglobin has higher affinity for O2 than hemoglobin...muscle GREAT at grabbing oxygen
changing the oxygen equilibrium curve a DECREASE in the oxygen affinity of a respiratory pigment has right and left shifts
decreased affinity hard to pick up, easy to drop off -decreased oxygen loading in lungs -increased oxygen unloading in tissue Hb + O2 <------- HbO2
Increased affinity easy to pick up, hard to drop off -increased oxygen loading in lungs -decreased oxygen unloading in tissue Hb + O2 -------> HbO2
curve shifts RIGHT if...
pH goes down CO2 goes up temp goes up HIGHER METABOLISM
curve shifts LEFT if...
pH goes up CO2 goes down temp goes down LOWER METABOLISM
raising right arm rapidly...
right shift for the right arm (the one that's raised...) -CO2 increases, H+ decreases, temp increases...right shift left shift for the left arm (the one that's NOT raised) -CO2 decreases, H+ increases, temp decreases...left shift
body does what with oxygen when one arm is raised?
drop off oxygen accordingly...
even with Bohr effect, saturation stays about the same...
hemoglobin about the same saturation...mess around with the steep part of the curve
why is too much O2 harmful?
slightly poisonous free radicals aging
oxygen equilibrium curve: ROOT effect (fish)
a decrease in the amount of O2 a respiratory pigment can bind at saturation can shift saturation DOWN advantage for fish: rapid unloading of gases into the swim bladder (altering pH can alter % saturation)
more than just waste! partial pressures at sea level (full humidity) -atmospheric: 30mmHg -alveolar: 40mmHg -venous: 45mmHg note: old air not completely expelled...not as big of a [ ] gradient as O2...we tend to favor keeping CO2 around
carbon dioxide levels in plasma
3ml CO2/100 ml plasma (10x levels of O2 in plasma) some also converted to carbonate some carbon dioxide binds to hemoglobin -affects O2 affinity to hemoglobin
Carbon Dioxide: Chloride Shift
the exchange of a chloride ion for bicarbonate across the red blood cell membrane allows rxn to be driven in one direction or the other enables the RBC to pick up and drop off high levels of CO2
the chloride shift has to do with...
CHARGE! we only want current for neurons and muscle cells without exchange, we generate current...bad! bicarbonate out, chloride in! bookkeeping of charge...
Chloride shift IN TISSUE
PREVENTS accumulation of bicarbonate in the cell...get it out! Bring In Cl- Increases CO2 PICKUP at tissues
Chloride shift IN LUNGS
brings bicarbonate BACK into cell to increase CO2 levels...kick out Cl- increases CO2 DROPOFF...get it out of body through expiration
produced by incomplete combustion competes with O2, binding to the heme group nearly IRREVERSIBLE binding to hemoglobin
CO and O2 are approximately the same...
skin tone & carbon monoxide...extra credit!
low levels of O2: blue appearance...hemoglobin w/o much oxygen...dark red color refracted by skin = blue when O2 binds to heme group...causes same bright red coloration since it's bound to hemoglobin...when refracted looks NORMAL...extremely dangerous!
won't let go like O2 does...so...
blood transfusions given because CO won't release from hemoglobin