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DNA TECHNOLOGY

Terms in this set (31)

Which enzyme(s) will produce a DNA fragment that contains the entire vgp gene (shown in red) and has "sticky ends"?
-BamHI
-HindIII
Which enzyme(s) would cut the human DNA shown in Part A on both sides of the vgp gene, but not inside the gene?
BamHI, HaeIII, and HindIII
Which enzyme (s) would cut the plasmid without disrupting the function of the ampR gene?
BamHI, EcoRI, and HaeIII
Which enzyme(s) would produce sticky ends when cutting both the human DNA and the plasmid?
BamHI, EcoRI, and HindIII
Which one restriction enzyme satisfies all three of the requirements listed above?
BamHI only
will grow only in medium without ampicillin
no plasmid
will grow in both media
the other 3 go here
What will be the sequence of nucleotides in the probe?
TCACACTG-GAATTCAT
In order to insert a human gene into a plasmid, both must _____
be cut by the same restriction enzyme
What enzyme forms covalent bonds between restriction fragments?
DNA ligase
This is a DNA fingerprint exhibiting samples from a victim, two suspects, and the crime scene. Which of these DNA fragments is common to both the victim and Suspect 1?
B
This is a DNA fingerprint exhibiting samples from a victim, two suspects, and the crime scene. Which of these DNA fragments is common to both the victim and Suspect 2?
C
Why is Suspect 1 considered more likely to have committed the crime than Suspect 2?
The crime scene sample contains DNA fragments from both the victim and Suspect 1.
Which of these DNA molecules is the shortest?
E
In gel electrophoresis DNA molecules migrate from _____ to _____ ends of the gel.
negative ... positive
What defect causes pituitary dwarfism?
Lack of growth hormone
What is genetic cloning?
A method to produce many copies of a gene
True or false? The use of growth hormone isolated from cadavers was banned mainly because there were not enough cadavers to supply the hormone.
False
Which of the following statements best describes the function of reverse transcriptase?
It makes complementary DNA (cDNA) from mRNA.
Which of the following steps would be performed last when screening a cDNA library?
Match the region of radioactivity on the filter with the corresponding plate.
How can large quantities of protein be produced from a bacterial colony containing the gene of interest?
Put a cDNA encoding the protein into a plasmid with a bacterial promoter sequence.
True or false? The term "sticky ends" refers to the overhanging ends on DNA that are generated by restriction enzymes, which can base pair with any DNA molecules that contain complementary sticky ends.
True
The unpaired nucleotides produced by the action of restriction enzymes are referred to as _____.
sticky ends
The sticky end of the DNA restriction fragment shown here will pair with a DNA restriction fragment with the sticky end _____.
-ACGT
What information can not be obtained from the sequence of a gene?
Whether the gene is methylated.
What is the polymerase chain reaction (PCR)?
A method to amplify a fragment of DNA.
True or false? Comparison of the sequences of the same gene across species can give some insight into the existence of a common ancestor with that gene.
True
True or false? The Taq enzyme is a type of DNA polymerase that allows researchers to separate the DNA strands during the annealing step of the PCR cycle without destroying the polymerase.
False
How many DNA molecules would there be after four rounds of PCR if the initial reaction mixture contained two molecules?
32
During which step in the PCR cycle are nucleotides used?
Extension
During which step in the PCR cycle do primers form bonds with a single-stranded template?
Annealing.
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