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microbiology lab practical 1
Terms in this set (64)
several individual organisms (especially of the same species) living together in close association
more than one type of culture growing in a sterile medium (multiple bacteria species)
microorganisms can be found everywhere, what is the word to describe this?
what do different colonies (sizes, shapes, colors) indicate?
what is the minimum amount of bacteria it takes to start a colony?
how many bacteria are present in one visible colony?
1 x 10 ^9
why is the plate incubated upside down?
to avoid condensation dripping on the bacteria and smearing it
what temperature is the plate incubated at?
37 degrees Celsius
why is the plate incubated at 37 degrees Celsius?
optimal bacterial growth
how do you hold a microscope when carrying it?
by the arm and the base
how do you find the total magnification?
ocular lens multiplied by the objective lens
4x is which lens?
10x is which lens?
40x is which lens?
100x is which lens?
what is always the ocular lenses magnification?
what is the total magnification for the scanning power?
what is the total magnification for the low power?
what is the total magnification for the high-dry lens?
what is the total magnification for the oil-immersion lens?
what do we do when we put the microscope away? (2)
put the scanning power down (4X)
put the stage all the way down
what are some safety precautions to take during lab?
wear closed toe shoes
tie hair back (open flame)
-what shape is a coccus bacteria?
-what shape is a bacillus bacteria?
-what shape is a spirillum bacteria?
-what is aseptic technique?
-what do you use aseptic technique on? (3)
-what is the rule to remember about this technique? (2)
-prevents contaminants during the experiment
-Inoculating loop, inoculating needle, and test tubes
-ALWAYS flame the loop before and after use, ALWAYS flame the open tube before you first collect a sample and before you replace the cap
-when the question is asking for "arrangement" or "shape" how do you write it?
-when the question is asking for "genus" or "bacteria" how do you write it?
-lower case "streptococcus" (arrangement) "coccus" (shape)
-using binomial nomenclature "Streptococcus" and underlined
how would you write the name of a bacteria?
capitalize the genus, lowercase the species, and UNDERLINE both words
what does sepsis mean?
what does asepsis mean?
when using aseptic technique, why is it important to flame items before and after, such as the open test tube and the loop before transfer of the bacteria?
to prevent contamination
when performing the smear prep, what is the important next to the last step, after air drying?
heat-fix the sample - waft it over the burner 3 times
what do we clean our bench with?
where are the fire extinguishers and safety blankets?
under her sink
-where do we dispose pipettes and Petri dishes?
-where do we dispose broken glass?
-where do we dispose test tubes?
-where do we dispose wet mounts?
what are the 2 purposes of heat-fixing?
kill the bacteria
adhere the bacteria to the slide
what is the primary stain?
crystal violet (purple)
-a positive result is from which stain?
-a negative result is from which stain?
what is the counter stain?
-what does a positive result look like?
-what does a negative result look like?
what is the decolorizer?
what molecule will make the gram-stain positive?
what color would a gram-positive cell be if you over washed with 95% ethanol?
what happens if you do not decolorize your gram-negative?
can turn out purple
what can result from too much heat-fixing?
unhappy cells, star patterns from dye, etc.
name the gram-positive species
Staphylococcus aureus, Bacillus cereus, Mycobacterium smegmatis, Streptococcus agalactiae
name the gram-negative species
Escherichia coli, Klebsiella pneumoniae
-what molecule is the Gram stain designed to differentiate in bacterial species?
-what color would a gram positive cell be if you over washed with 95% ethanol?
-what would happen if you do not decolorize your gram negative cell?
-It could turn purple
Acid Fast Stain:
-what is the primary stain?
-what is the decolorizer?
-what is the counter stain?
what went wrong with your staining technique if you acid-fast species came out blue instead of pink? (2)
too much acid alcohol OR water bath not hot enough
Acid Fast Stain:
-what color is positive? what dye?
-what color is negative? what color?
-blue, methylene blue
-what is the primary stain?
-what is the counterstain?
-Is there a decolorizer?
what organism is an acid fast positive?
what molecule makes an acid-fast positive?
what are the two techniques used to determine motility?
-what color do endospores stain? what dye?
-what color do the vegetative cells stain? what dye?
-green, malachite green
Is there a decolorizer in the spore stain?
No, only a water rinse
what organism forms spores?
what went wrong with the procedure if the Bacillus species' do not stain?
did not heat properly
decolorize on accident (there is no decolorize)
when making a wet mount what is beneficial when putting the coverslip on? why?
trying to get an air bubble, because the bacteria will not enter the air bubble so they will gather around it
-when done with motility testing, what should the stage of the microscope be cleaned with?
-where should the slides with cover slips go?
-what color will the agar turn if the bacteria are motile?
-what motion do motile bacteria do?
-what motion do non-motile bacteria do?
-what media do we use?
-straight run and tumble
-name a motile species
-name a non-motile species
why would a non-motile species look motile?
accidentally move inoculating needle instead of going straight down
KNOW TO WRITE BINOMIAL NOMENCLATURE, know how to spell everything, know powerpoint
THIS SET IS OFTEN IN FOLDERS WITH...
cell membrane & cellular Transport
Chapter One Micro-Biology
Acid Base Balance
Chapter 4 Micro- Biology
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