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How is gene expression regulated? In 1940, Francois Jacob and Jacques_____ found that E. coli can digest _____ all the time. They can also digest lactose sometimes. When E. coli are grown in glucose, a _____ binds to DNA preventing RNA polymerase from transcribing the lactose digesting enzymes. When E. coli are grown in lactose, the lactose molecule binds to the repressor protein. RNA polymerase is free to transcribe the lactose-digesting proteins.