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Micro Exam 2 CH. 5-7
Terms in this set (70)
The photosynthetic pathway used by plants in the light reaction resembles which of these pathways?
Electron Transport Chain
An plant cell is an example of which of the following groups of living organisms based on metabolism?
Which of the following compounds is NOT an enzyme?
Glucose + Galactose --> Lactose
The above reaction is an example of
During any enzymatic reaction, which of the following molecules is changed ?
Oxalacetate and alpha-ketoglutarate form the carbon skeletons for 2 families of essential amino acids. Which pathway are these 2 essential intermediates found?
How many high-energy bonds are in an ATP molecule ?
Fatty acids are broken down and converted to AcCoA in which pathway?
Biochemically enzymes are which type of macromolecule:
Enzymes can be denatured by:
- Extreme pH changes
- Organic chemicals like formaldehyde
- High iconic salt concentrations
- High temperatures
The synthesis of large molecules from a smaller molecules is called:
How many ATP does it take to start glycolysis?
The synthesis of purines and pyrimidines for DNA and RNA synthesis used which of these pathways?
- Entner Doudoroff
- Pentose Phosphate
- Krebs cycle
An apoenzyme that loses its coenzyme subunit will be non-functional.
Glycolysis is primarily an anabolic pathway.
Kreb's Cycle can be both catabolic and anabolic depending where the carbons go.
Every FADH2 that enters the electron transport chain carries enough energy to synthesize 2 ATP molecules.
Enzymes are classified into 6 groups based on which type of chemical reactions they catalyze.
Coenzymes along with the substrates are consumed in enzymatic reactions.
Loss of electrons
Electron Transport Chain
One end production of fermentation
The time it takes one bacterium to grow in size and divide into 2 is called the :
In performing plate counts, what are the 2 numbers used to determine which plates to count?
30 and 300
All bacterial species grow at the same rate.
In which phase of a growth curve are the bacteria rapidly dividing and dying at about the same rate?
Which of the following is NOT a direct method of counting bacteria?
The most common way to monitor bacterial growth using an indirect technique is by :
A quick way to get an accurate count of the number of bacteria in a culture is by :
Direct microscopic count
The biosafety level (BSL) for most introductory microbiology laboratories is
The biosafety level (BSL) for most clinical and hospital laboratories is
What is the pore size of MOST biological membranes used for sterilization by filtration?
Which of the following pairs is mismatched?
Thermophile growth at 37°C
Which of these Genera consists of anaerobes?
3 cells with generation times of 60 minutes are inoculated into a culture medium. How many cells are there after 5 hours?
You have a sample from a culture of growing bacteria and you need to immediately know the exact number of bacteria in the sample. Which is the best way below to produce that information?
Direct microscopic count
Reducing media is used for the cultivation of capnophiles.
Agar is used as a solidifying agent in microbiological media since few bacteria can degrade it.
1:10; 1:100; 1:1000; 1:10,000 is an example of a serial dilution.
Laboratory cultivation of obligate anaerobes requires reducing media or special growth chambers filled with inert gases.
Hypotonic means there is less water in the solution than in the cells .
Can't grow in presence of oxygen
Toxic oxygen radical
Initiates growth of a culture
The destruction of ALL life is defined as :
How do disinfectants work ?
- By destroying cell membranes.
- Destroying DNA and RNA.
- Damaging proteins.
The proper pressure, temperature, and time of autoclaving is:
15 psi, 121 C, for 15 min.
Which of these can affect disinfection of a specific bacterium by a chemical ?
- The bacterial load.
- Characteristics of the bacterium
- Presence of organic material.
- Time of exposure
Wet heat can sterilize faster than dry heat.
Which of the following is a limitation of the autoclave?
It cannot be used with heat-labile materials.
This term is used to describe the destruction of all pathogenic organisms.
UV light kills microorganisms by damaging their:
Which of the following pairs of terms is mismatched?
Bacteriostatic kills vegetative bacterial cells.
The clear area around a antibiotic disk on a spread plate is called the :
Zone of inhibition
Which of the following chemical agents is used for sterilization of plastics?
Which of the following does not destroy bacterial endospores ?
Solutions of heat sensitive chemicals such as vitamins, antibiotics, and serum components could best be sterilized by:
The time, pressure, and temperature of an autoclave was designed to specifically destroy :
To eliminate most bacteria, what is the largest size pore found in membrane filters?
Ultraviolet light (UV) causes breaks in DNA strands.
Does the presence of organic materials affect the efficacy of most disinfectants?
Yes, it can
HEPA refers to sterilization by filtration.
Autoclaving is the most effective method of moist heat sterilization.
Quaternary ammonium salts are good cleansers as well as disinfectants.
Destruction of vegetative pathogens on inanimate objects.
Kills endospores C. botulinum
Lowers the microbial load on objects
Destruction of all life
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