Upgrade to remove ads
Only $2.99/month

BISC 260 LAB FINAL

Terms in this set (87)

Oxidase Test: original color
N/A
Oxidase Test: positive test color
purple/dark blue within first few seconds
Oxidase Test: negative test color
no color
Oxidase Test: reagent used
oxidase reagent
Oxidase Test: enzyme tested for
cyochrome oxidase
Oxidase Test: general purpose
-used to identify bacteria that produce cytochrome c oxidase, an enzyme of the bacterial electron transport chain
-takes 1 minute and avoids false negative results and the necessity to grow the bacteria in a general purpose medium
-differentiate between non-enteric and enteric organisms
Catalase Test: original color
clear
Catalase Test: positive test color
clear with bubbles
Catalase Test: negative test color
clear without bubbles
Catalase Test: reagent used
hydrogen peroxide
Catalase Test: enzyme tested for
catalase
Catalase Test: general purpose
-test the presence of catalase, an enzyme that catalyses the release of oxygen from hydrogen peroxide
-used to differentiate those bacteria that produces an enzyme catalase, such as staphylococci, from non-catalase producing bacteria, such as streptococci
Methyl Red Test: original color
Yellow
Methyl Red Test: positive test color
red
Methyl Red Test: negative test color
Yellow (no change)
Methyl Red Test: reagent Used
mixed acid
Methyl Red Test: enzyme tested for
...
Methyl Red Test: general purpose
detects the production of sufficient acid during the fermentation of glucose and the maintenance of conditions such that the pH of an old culture is sustained below a value of 4.5, as shown by the color change
Vogues-Proskauer Test: original color
yellow
Vogues-Proskauer Test: positive test color
pink or red
Vogues-Proskauer Test: negative test color
yellow or copper at the surface (no change)
Vogues-Proskauer Test: reagent used
distilled water, ethanol, potassium hydroxide
Vogues-Proskauer Test: enzyme tested for
acetoin
Vogues-Proskauer Test: general purpose
-used for identification of Enterobacteriaceae
-based on the detection of end products from the metabolism of glucose
-based on the detection of acetoin, and its detection is achieved by the addition of various reagents, that leads to red colored end products
Indole (SIM) Test: original color
yellow
Indole (SIM) Test: positive test color
red layer
Indole (SIM) Test: negative test color
yellow (no change)
Indole (SIM) Test: reagent used
Kovac's reagent
Indole (SIM) Test: enzyme tested for
trytophanase
Indole (SIM) Test: general purpose
detection of sulfide production, indole formation, and motility
Starch Media: selects for
...
Starch Media: differentiates between
organism production of exoenzymes
Starch Media: what's isolated
clearing around bacterial growth
Starch Media: componets
...
DNase Media: selects for
-hydrolyzing of DNA
-utilizing DNA as a source of carbon and energy for growth
DNase Media: differentiates between
-production of deoxyribonuclease (DNase) or not
-S. aureus from other Staphylococci
DNase Media: what's isolated
DNase positive organism (green to colorless)
DNase Media: components
...
Motility Test: original color
N/A
Motility Test: positive appearance
red diffuse growth radiating from the stab line
Motility Test: negative appearance
red color only along stab line
Motility Test: reagent used
Triphenyltetrazolium Chloride (TTC)
Motility Test: testing for
motility
Motility Test: general purpose
to detect bacterial motility, which is an important differential characteristic of Enterobacteriacae and other groups
Triple Sugar Iron (TSI) Test: original color
medium red
Triple Sugar Iron (TSI) Test: reagent used
none
Triple Sugar Iron (TSI) Test: enzyme tested for
Thiosulfate reductase, which causes a positive reaction for hydrogen sulfide production
Triple Sugar Iron (TSI) Test: general purpose
-differentiate bacteria on the basis of glucose, lactose, sucrose fermentation and sulfur reduction
-differentiate members of Enterobacteriaceae and distinguish them from other gram negative rods
Triple Sugar Iron (TSI) Test: why red slant
means glucose fermentation
Triple Sugar Iron (TSI) Test: why yellow slant
means glucose and lactose/sucrose fermentation
Triple Sugar Iron (TSI) Test: why red butt
means peptone catabolized aerobically and anaerobically with alkaline products
Triple Sugar Iron (TSI) Test: why yellow butt
means proteins catabolized aerobically with alkaline products (reversion)
Triple Sugar Iron (TSI) Test: why black butt
means sulfur reduction and fermentation; look at slant color to determine which carbs have been fermented
Triple Sugar Iron (TSI) Test: why gas formed butt
means fissures in medium or will lift agar off bottom of the tube
Oxidation Fermentation Glucose (O/F) Test: why use mineral oil
-to promote anaerobic growth and fermentation
-creates an environment unsuitable for oxidation because it prevents diffusion of oxygen from the air to the media
Oxidation Fermentation Glucose (O/F) Test: anaerobic results
-alkaline (green)
-acid (yellow)
-alkaline (green)
Oxidation Fermentation Glucose (O/F) Test: aerobic results
-acid (yellow)
-acid (yellow)
-alkaline (green)
Oxidation Fermentation Glucose (O/F) Test: result of alkaline (green) anaerobic and acid (yellow) aerobic
means oxidative
Oxidation Fermentation Glucose (O/F) Test: result of acid (yellow) anaerobic and acid (yellow) aerobic
means fermentative
Oxidation Fermentation Glucose (O/F) Test: result of alkaline (green) anaerobic and alkaline (green) aerobic
means non-saccharolytic (glucose not metabolized)
Eosin Methylene Blue (EMB) Media: selects for
gram negative organisms
Eosin Methylene Blue (EMB) Media: differentiates between
growth inhibited by dyes or not to show fermentation
Eosin Methylene Blue (EMB) Media: what's isolated
fecal coliforms
Eosin Methylene Blue (EMB) Media: components
digest of gelatin, lactose, eosin Y dye, and methylene blue dye
MacConkey Agar Media: selects for
gram negative bacteria
MacConkey Agar Media: differentiates between
pH by neutral red dye
MacConkey Agar Media: what's isolated
gram negative bacteria
MacConkey Agar Media: components
bile salts and crystal violet
Mannitol Salt Agar Media: selects for
Stahylococcus
Mannitol Salt Agar Media: differentiates between
Staphylococcus aureus from other Staphylococcus species
Mannitol Salt Agar Media: what's isolated
growth of Staphylococcus
Mannitol Salt Agar Media: components
carbohydrate mannitol, 7.5% NaCl, phenol red pH indicator
Hektoen Enteric Agar Media: selects for
gram positive organisms
Hektoen Enteric Agar Media: differentiates between
color of colonies and agar (yellow to salmon/pink); coliforms
Hektoen Enteric Agar Media: what's isolated
Salmonella and Shigella species from other gram negative enterics
Hektoen Enteric Agar Media: components
bile salts, bromthymol blue dye, and fuchsin acid
Citrate Media: selects for
citrate positive organisms
Citrate Media: differentiates between
the ability of organisms to utilize citrate as a carbon source
Citrate Media: what's isolated
citrate positive organisms if medium turns blue
Citrate Media: components
sodium citrate as a source of carbon, ammonium dihydrogen phosphate as nitrogen, and bromthymol blue pH indicator
Blood Agar Media: selects for
gram positive organisms and hemolytic reaction
Blood Agar Media: differentiates between
hemolysis reactions
Blood Agar Media: what's isolated
bacteria that does not grow well on other media
Blood Agar Media: components
casein, digest of animal tissue, beef extract, yeast extract, corn starch, and sheep blood
Blood Agar Media: alpha-hemolytic
-partial lysis of the RBCs to produce greenish-gray or brownish discoloration around a colony
-reduction of RBC hemoglobin to methemoglobin in the medium surrounding the colony
Blood Agar Media: beta-hemolytic
complete lysis of RBCs causing a clearing of blood from the medium under and surrounding the colonies
Blood Agar Media: gamma-hemolytic
-no hemolysis of RBCs
-no change in the medium under and surrounding the colonies
THIS SET IS OFTEN IN FOLDERS WITH...