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Virology Lab Quiz
Terms in this set (25)
SHRV Optimal Temp
enveloped, non-segmented, negative sense single stranded RNA
How many genes does SHRV have
progressive multifocal leukoencephalopathy
Cytolytic destruction of myelin-producing glial cells causing demyelination. Caused by JCPyV. High risk for immunosuppressed people.
Small, nonenveloped, dsDNA virus. bidirectional. regulatory region (RR), early genes (T-Ag), late genes (viral capsid proteins). Three viral capsid proteins in the late gene region: VP1,VP2,VP3. VP3 serves as the receptor attachment site.
JCPyV Infection sites
brain (glial cells) and kidneys.
Human fetal glial cells comprised of mostly astrocytes. Transformed with SV40 Large T Antigen. Highly permissive to JCPyV infection.
cytopathic effect (CPE)
a visible effect on a host cell, caused by a virus, that may result in host cell damage or death. Morphological effects: syncytia, inclusion bodies, swelling, rounding, lifting.
Physical quantitative assays
electron microscopy and hemagglutination assay
tissue culture infective dose 50%. the viral dilution that would yield infection in exactly 50% of samples.
(# of dilutions where CPE > 50%) + ((% above 50% - 50%) / (% above 50% - % below 50%))
detects viral proteins. visualizes single infectious cells with immunofluorescence. measures titer in fluorescent focal units.
(# of infected cells x dilution factor) / mL used to infect
minimal essential media
Dulbecco's Modified Eagle's Medium
paraformaldehyde. fixes cells
BSL-2, can infect human cells and cause minor health issues.
method used to measure the infectivity of the virus. one plaque = one infectious unit. Titer is measured in PFU/mL (plaque forming unit)
HEK 293A cells
human embryonic kidney cells.
can trigger an immune response to gluten (celiac disease)
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