In chymotrypsin, the catalytic triad is made up of which three amino acids?
Asn, His, Ser
Asp, His, Ser
Asp, Asp, Ser
Asn, His, Cys
Asp, His, Cys
Peptide hydrolysis by chymotrypsin involves several steps. Descriptions of some of the steps are given here. Place the steps in the correct mechanistic order (some additional steps may be occurring in the mechanism but aren't described here).
i. The amine group of the peptide leaves the enzyme.
ii. The carboxylic acid product leaves the enzyme.
iii. Tetrahedral intermediate is stabilized by the oxyanion hole.
iv. The oxygen atom of Ser makes a nucleophilic attack on the carbonyl carbon of the target peptide bond.
v. Histidine acts as a general acid.
vi. Water enters the active site.
vii. The acyl-enzyme is generated.
iv, iii, vii, ii, vi, v, i
iv, vii, i, v, vi, iii, ii
v, iv, iii, vii, i, vi, ii
iv, iii, vii, i, vi, v, ii
iv, vii, iii, i, vi, v, ii