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Bio Exam 3 Review ?'s

Terms in this set (14)

The distance a fragment of DNA travels on an Electrophoretic GEL is directly correlated with
its total number of nitrogenous bases; whether it's single or double stranded.
One would expect an equivalent length/fragment of RNA to travel further than an equivalent length of DNA on a GEL
because DNA is generally double stranded and RNA is generally single stranded.
DNA that is produced from RNA is called
cDNA
Which of the following methods is used to determine the base sequence of DNA?
dideoxy sequencing
Which enzyme produces cDNA?
reverse transcriptase
Which of the following is/are requirements for PCR?
Only possible when DNA sequence info surrounding gene is available; primers that match sequences on both sides of the gene needed; DNA polymerase
Which of the following allow scientists to conduct DNA fingerprinting (aka DNA profiling)?
short tandem repeats, variable number tandem repeats
How many potential reading frames are there for a DNA molecule?
6 (3 per strand, 2 strands, 2x3=6)
Which of the following explain(s) why looking for ORFs (open reading frames) in eukaryotes is more complicated?
presence of introns, very long ORFs (impossible to scan for)
What is an example of molecular fossils?
human transposable elements
Explain duplication and divergence (has to do with unequal crossing over during meiosis)
Mutations in duplicated segment leading to new protein function; proceeds unequal crossing over (misaligned chromatids), in which one sister chromatid has duplication of repeated sequence. This leads to the formation of gene families (genes with similar functions)
Which of the following are features of genetic markers?
...
One common method for preparing large quantities of a particular gene is

electrophoresis
BAC
nucleic acid hybridization
PCR
PCR
Cas9 uses

an SNP to identify a compliment of DNA.
a guide RNA to identify a complementary DNA.
an endonuclease to identify a complementary DNA.
none would apply
a guide RNA to identify a complementary DNA.
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