Upgrade to remove ads
Only $2.99/month

ENDO Exam 2 - Immuno

Terms in this set (62)

What two mucins do female epithelial cells secrete (w/in the reproductive tract)?
MUC4, MUC5
What 7 mucins do male epithelial cells secrete (w/in the reproductive tract)?
MUC1, MUC3, MUC4, MUC13, MUC15, MUC17, MUC20
What is the ONE mucin that both males & females have in common—which epithelial cells secrete (w/in the reproductive tract)?
MUC4
Upper reproductive (endocervix, Fallopian tubes, uterus) tract in females is lined with what type of cell type? Each cell is joined by what?
Columnar epithelial (single layer); tight junctions
What is one major microorganism (microbiome) in the female reproductive tract?
Lactobacillus
Lower reproductive (ectocervix and vaginal tract) tract in females is lined with what type of cell type?
Stratified squamous epithelium
Name the 4 major types of innate immune cells found in the female reproductive tract?
Macrophages, DCs, Neutrophils, NK cells
What type of innate immune cells makes up the majority within the female reproductive tract?
Macrophages
What is the dominant adaptive immune cell type in the female reproductive tract?
CD8 T cells
What are the two adaptive immune cell types in the female reproductive tract?
CD4 & CD8 T cells (CD8 T cells more frequent in upper reproductive tract)
B cells in the female reproductive tract that are associated with lymphoid aggregates primarily express what type of CD marker?
CD5
What immunoglobulin is the dominant one within the female reproductive tract? What is also a resident Ig within the FRT?
IgG; IgA (resident plasma cells)
From what day to what day (of the ovulation cycle) are the NK cells most active in the female reproductive tract?
From day 7 and on
From what day to what day (of the ovulation cycle) are the pIgA's MOST active in the female reproductive tract?
From day 1 to day 14
From what day to what day (of the ovulation cycle) are the pIgA's LEAST active in the female reproductive tract?
From day 14 and on
From what day to what day (of the ovulation cycle) do the CD 8 T cells have HIGH cytolytic potential within in the female reproductive tract?
From day 7 to day 14
From what day to what day (of the ovulation cycle) do the CD 8 T cells have LOW cytolytic potential within in the female reproductive tract?
From day 14 to day 28
From what day to what day (of the ovulation cycle) are there a HIGH LEVEL of macrophages within in the female reproductive tract?
From day ~16 to day 25
From what day to what day (of the ovulation cycle) are there a HIGH LEVEL of neutrophils within in the female reproductive tract?
From day 25 and on
During the ovulation period, about what day does the body temperature go up? What is happening during this period - when the temperature rises?
About day 14; ovulation
During the ovulation cycle, what day does estradiol peak?
Day 14
Production of what female sex hormone favors Th2 cytokine production?
Progesterone
Production of what female sex hormone (in LOW concentrations) will INCREASE levels of TNF-alpha, IL-6 & IL-1?
Estrogen
Production of what female sex hormone (in LOW concentrations) will INCREASE levels of leukocyte migration to sites of inflammation?
Estrogen
Production of what female sex hormone (in HIGH concentrations) will INCREASE levels of IL-4, IL-10, TGF-Beta?
Estrogen
Production of what female sex hormone (in HIGH concentrations) will DECREASE levels of cell-mediated immunity and CTL Acttivity?
Estrogen
What type of contraceptive (example: DMPA - DepoProvera) will decease following immune responses:
↓ antibody production
↓ IFN-Beta production
↓ NK cell function and FcR expression on monocytes
↓ CTL activity/performing expression
Progesterone-based contraceptives
What type of contraceptive will decrease the following immune responses:
↓ Inflammatory effects
• autoimmune disease
• inflammatory bowel disease
• other
Estrogen-based contraceptives
What type of epithelial cells line the urethra of the male reproductive tract?
Single columnar epithelial cells
What type of epithelial cells line the male reproductive tract - AFTER the urethra toward the outside world?
Non-keratinized stratified squamous epithelium
What protein in males helps seal the tight junctions between the blood-testis barrier?
Claudin 3
Within the male reproductive tract, what two gonadatropins help regulate macrophages locally in this region?
LH & FSH
What 3 antimicrobial peptides are constitutively expressed in the mucosal
epithelia and immune cells within the male reproductive tract?
SLPI, lactoferrin, & lysozyme
What are the major regions (within the male reproductive tract) where there is NO TLR expression on epithelium and immune cells?
Rate Testis, Foreskin & Vas Deferens
What are the 4 major TLR's expressed within the male reproductive tract?
TLR-1, -3, -8, -9
What is the ONLY TLR expressed on the immune cells of these tissues:
- testis
- efferent ducts
- epididymis
- seminal vesicles
TLR-1
In the male reproductive tract - briefly describe what type of protein is TLR-1?
Bacterial Lipoprotien
In the male reproductive tract - briefly describe what type of protein is TLR-3 & TLR-8? Where within the male reproductive tract is this expressed?
TLR-3: dsRNA; TLR-8: ssRNA; epithelial cells of prostate
In the male reproductive tract - briefly describe what type of protein is TLR-9? Where within the male reproductive tract is this expressed?
unmethylated CpG-rich DNA; immune cells of epithelial cells of penile urethra
What is this describing: property of certain sites or tissues whereby immune responses are limited or prevented?
Immune Privilege
What are the two major macrophage types within the male reproductive tract?
(ED1) M1 & (ED2) M2
Out of the two major macrophage types within the male reproductive tract, which one responds to infections and how do they respond? What percent does this take up within both macrophage types?
(ED1) M1 by initiating inflammation; 20%
Out of the two major macrophage types within the male reproductive tract, which one helps maintain immune privileges and how do they do this? What percent does this take up within both macrophage types?
(ED2) M2 by:
• secreting anti-inflammatory cytokines
• they have reduced ability to produce pro-inflammatory cytokines
• they support Leydig cell steroidogenesis

(ED2) M2 make up 80%
Out of the 2 types of macrophages within the male reproductive tract, which one is PROINFLAMMATORY? Which one is ANTI-INFLAMMATORY?
(ED1) M1; (ED2) M2
What macrophage type (within the male reproductive tract) is this describing:
(ED2) M2
What macrophage type (within the male reproductive tract) is this describing:
(ED1) M1
What are the 3 major immunoregulatory factors in semen?
1. TGF-Beta
2. Antibodies
3. Seminal Prostaglandins
What type of male reproductive tract immunoregulatory factor present in semen is this describing:
- inhibit function and clonal expansion of NK and T cells
- polarize CD4 T cells toward Th2 phenotype
- promotes switch: IL-12 to IL-10 production in DC's
Seminal Prostaglandins
What type of male reproductive tract immunoregulatory factor present in semen is this describing:
- activates/recruits PMN's, Macrophages and DC's
- elicits a transient pro-inflammatory response
TGF-beta
What type of male reproductive tract immunoregulatory factor-antibodies are present in semen?
IgA, IgG, IgM
What type of male reproductive tract immunoregulatory factor present in semen will inhibit activation and clonal expansion of NK and T cells?
Seminal Prostaglandins (PGE)
What type of male reproductive tract immunoregulatory factor present in semen will activate/recruit PMN's, Macrophages and DC's?
TGF-beta
CD56 (bright)/CD16 (dim); CD16 = high toxicity
What is unique about the uNK Cell markers? What specific marker on normal NK cells has a link to the level of high toxicity?
CD56 (dim)/CD16 (bright)
What kind of markers do peripheral NK cells have?
KIR2DL4; activating
What soluble uNK cell receptor binds to HLA-G on the MHC molecule? Is this an ACTIVATING or DEACTIVATING signal?
High level of cytokine secretion
What is the HLA-G receptor associated with when activated on the uNK Cells?
LILRB1; deactivating
What surface uNK cell receptor binds to HLA-G on the MHC molecule? Is this an ACTIVATING or DEACTIVATING signal?
Tr1 (suppressor cell); secrete IL-10; increase tolerance
What kind of cell does soluble HLA-G induce-other than control EVT invasion within the placenta? What do these cells secrete? Overall, what is the net reaction?
FasL; EVT Cells;
What kind of ligand does soluble HLA-G induce-other than control EVT invasion within the placenta? What cell expresses this ligand?
CD 8 T cells; CD 8 T cells die
FasL is expressed on EVT Cells. What cell expresses the FasR (receptor)? When both (FasL-FasR) are bound, what cell is stimulated to die?
IDO
What EVT cell intracellular enzyme has this effect when activated: ↓ Tryptophan which leads to ↑ Hyper-activation of Treg Cells and ↑ effector cells = OVERALL leads to an increased tolerenegic environment?
IDO
What EVT cell intracellular enzyme has this effect when activated: metabolizes Tryptophan into KYN?
YOU MIGHT ALSO LIKE...