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micro lab 224 midterm luquette
Terms in this set (63)
PDA (potato dextrose agar)
selective media for fungi and bacteria
PCA (plate count agar)
general, nutritional medium designed to grow microorganisms that normally live in a dilute environment; this medium is commonly used to determine the number of microorganisms in food and water samples
microorganisms in the environment are _______
what percent of agar is needed to make media hard?
nigrosine, india ink, anionic
_______ or ______ is the dye used in a negative stain, this dye is _______
_______ bacteria was used in the negative stain that was done in class. this bacteria was _______ shaped.
what is the stain done in class that requires a small amount of ink and bacteria being put at the edge of slide and then smeared across thinly with second slide?
crystal violet, safranin, methylene blue
what are some cationic dyes? these dyes are considered to be?
what are some anionic dyes? these dyes are considered to be?
spore that is located at the end of the cell
endospore that is located between the center and the end of the cell
spore located in the middle of the cell
vegetative cell-sporulation-free spore (dormant, resistant in this stage)-germination
spore cycle stages
the metabolically active and growing stage of endospore cycle (sometimes the bacteria is stressed in some manner and will start the spore cycle here)
in this stage of the spore cycle the dna of the cell is copied and placed in the spore structure
the spore is now formed and the vegetative cell disengrates and the spore is released into the enviroment, can lay dormant and endure for many years in this stage of the spore cycle
stage of spore cycle when conditions are favorable the spore coat is discarded and the dna reproduces to create new bacterial organism and the cycle starts all over again
malachite green (1 min)
safranin (1 min)
heat (5 min)
_______ and _______ was used in the schaeffer-fulton ________spore stain. the bacteria was gram ______ and ______ shaped. the primary stain was ________ and the secondary stain was _______. the mordant used was _____
gram positive bacteria
Bacteria that have a thick peptido glycan cell wall, and no outer membrane. They stain very darkly (purple) in Gram stain. has teichoic acid
type of bacteria that stain red/pink with Gram stain and have a thin cell wall with an outer membrane
alcohol (3-5 sec, quick!)
_____ and _____ were used in the gram stain done in class. the primary dye used was ______ and the countwerstain was _______. the decolorizer was _______ and the mordant was _______
what was the gram negative bacteria involved in the gram stain done in class? what was its shape?
what was the gram positive bacteria used in the gram stain doen in class? what was its shape?
what is another name for differential spore stain?
range size shape
what can a simple stain tell you about a bacteria?
kill bacteria (sterilize) coagulate proteins open pores (to take dye, stain)
what are three reasons to heat fix bacteria before you stain?
acid fast stain
A staining procedure for identifying bacteria that have a waxy cell wall.
________ is the bacteria used in (kinyoun) acid fast stain. the primary stain is _______ and the counterstain was ________. the decolorizer used was _______
stain used in the simple stain procedure
500 nm/2 x n.a
resolving power equation
comma shaped bacteria
what shape bacteria is this?
what is the arrangement of bacteria
what is the arrangement of this bacteria
what is the arrangement of this bacteria?
what are the three types of motility
run and tumble method
movement done by bacteria that have several flagella
Run and Tumble-Flagella
types of motility of bacteria
Facultative Motile Organism
tube is cloudy from side to side, stab line is obscured, and the top is completely overgrown
Facultative Non-Motile Organism
Tube is cloudy only along stab line and top has only mound of growth
Aerobic Motile Organism
Tube is cloudy from edge to edge only where oxygen is present and top is completely overgrown.
What bacteria was facultative motile in the motility stab done in class? (Tube was cloudy)
What bacteria was facultative non motile in the motility stab done in class (bacteria only grew on the stab line)
What bacteria was aerobic motile in the motility stab done in class (cloudy only at the top where oxygen is)
What lenses should you not use the COARSE FOCUS KNOB with??
poisoned by oxygen
can live with or without oxygen
What cell does the oil immersion lense look at?
ocular lens (10x magnification)
Eyepiece of a microscope
Holds and turns the objectives into viewing position
swivels to permit eyepiece housing to rotate
stage adjustment knobs
used to mechanically move the stage side to side, front to back
lamp intensity dial
Used to adjust light intensity. Normally set between 8-10.
used to adjust both intensity and contrast of light on microscope
scanning lens 4x
lower power lens
high power dry lens 40x
high power oil immersion lens 100x
oil must be used to focus properly
what is number 11
what is number 12?
coarse focus knob
moves stage up and down
fine adjustment knob
Moves the stage slightly to sharpen the image
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