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Experiment 12: Soil Microbes
Terms in this set (16)
Symbiotic relationship with legumes
Rhizobium and Bradyrhizobium
An iron-containing protein in red blood cells that reversibly binds oxygen.
Gives nodules their pink color
Rhizobium trifolii lives in symbiosis with
Members of rhizobium genus are
Gram negative, aerobic rods
Examples of free living anaerobic nitrogen-fixing bacteria
Clostridium and Desulfovibrio
Examples of free living aerobic nitrogen-fixing bacteria
Azotobacter and Azomonas
Nitrogen fixation requires two enzymes
dinitrogenase and dinitrogenase reductase
Uses heterocyst to compartmentalize nitrogenase
What does Azotobacter use to protect it form oxygen inactivation?
Third protein; produces an O2 retarding alginate capsule
Azotobacter was discovered by
Beijerinck discovered Azotobacter using
an enrichment culture technique
To enrich Azotobacter, the soil sample is added to a broth medium that contains why as an energy and carbon source?
What is not added to broth to enrich Azotobacter?
Why is nitrogen omitted?
Only those who can use gaseous nitrogen will grow (like Azotobacter)
Azotobacter can readily use what as a carbon source?
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