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Practice test 3 genetics
Terms in this set (26)
what is required for when Phage are mixed at high multiplicity with K cells and plated at low multiplicity on K cells.
complementation plus recombination
what is required for when Phage are mixed at high multiplicity with K cells and plated at low multiplicity on B cells
Two T4 rII mutants were infected into B cells at high multiplicity. The lysate was collected,
diluted 100 times, and 0.01 ml was plated on B cells at low multiplicity. 1000 plaques
resulted. 0.01 ml of the undiluted lysate was plated at low multiplicity on K12 cells. 200
plaques resulted. What is the distance between the two mutants?
A human chromosome contains
multiple origins of replication
For DNA synthesis, DNA polymerase III requires:
dNTP's, primer, template
DNA polymerase I adds nucleotides onto what group of the growing DNA strand?
The 3' hydroxyl group
For the discontinuous strand, DNA polymerase III synthesizes DNA
away from the replication fork
an RNA molecule
RNA differs from DNA in that RNA has
a 2' OH, and a Uracil
What DNA synthesizing enzyme uses RNA as a template?
Translation is the process of:
Transcription is the process of
RNA processing involves
capping, poly adenylation, splicing
Two mutations in the trpA gene of E.coli result in a change in the same amino acid. These
Cannot recombine to give a wild type protein.
Can recombine to give a wild type protein.
The carboxyl terminus of a protein corresponds to the:
3' end of the mRNA
The tertiary structure of a protein is encoded by the
DNA base pair sequence
A hydrophobic amino acid is likely to be located in
The interior of a protein
The splicesome joins:
A protein had the amino acids ile-cys at codons 10 and 11. A deletion of a single base
occurs in the ile codon that changed the protein so that it now reads tyr-ala at codon 10 and
11. The original ile codon must have been:
A missense mutation results in
An amino acid substitution
A point mutation results in a nonsense mutation in a gene. The original amino acid
encoded at the mutant codon could have been
A tRNA in E. coli charged with asp would have the anticodon
Initiation of protein synthesis in E. coli requires:
AluI cleaves DNA at a GTAC sites. A bacterial chromosome is 2.56x106
bp. Which is the
best estimate of the number of fragments that results from AluI cleavage?
In forensic analysis, STRs are analyzed by
PCR and gel electrophoresis
The goal of cloning is to obtain what for a gene?
purification, expression, amplification
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