Cell based therapies 3 (PB)

Terms in this set (18)

-Can blend solution of polymer with something that is adhesive to cells (biologically/therapeutically functional agents) e.g. collagen and then electrospin so that cell adhesion species on the surface to cells to grow on

-can also chemically treat pre-formed scaffold just to modify the surface:
-electrospun nanofibres undergo wet or chemical treatment to change the chemical functionality of the surface
-then can either attach biocompatible nanofibres or immoblise and attach a protein/enzyme/growth factor/drug=biologically or therapeutically functionalised nanofibres

-lots of ways to achieve scaffold modification, in particular polyesters (e.g. PGLA) can be modified using NaOH or primary amines:
[-e.g. think of ester bond, NaOH breaks that bond --> get carboxylic acid + hydroxyl group
-can form group that has available functional groups that can attach things to surface with covalent bond]
-subsequent reaction with coupling reagents can be used to attach bioactive motifs (or whole proteins) to scaffolds to make them more adhesive to cells (or can use fragments of proteins)
-mineralisation of scaffolds can be achieveved with simulated body fluid (SBF) (to make sure integrate with body well and that osteoblasts will grow on them - e.g. treat PLGA with NaOH, introduces carboxyl acids and hydroxyls = can act as nucleation sites for the formation of hydroxyapatite crystals=incubate with SBF=form hydroxyapatite for bone)
-functionality can be designed in e.g. design polymer to have pendant backbone RGD groups
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