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Why is kinetics important?
the rate of enzmyes with different substraes is imporatnat for drug desgn (in the presences of inhibitor)
What does the michaelis-mention eq do?
explains what you see experimentally when you do an enzyme catalzyed rxn
What does the horizontal slope of the hyperbola graph of MandM Equation stand for?
Vmax meaning its going as fast as it can go and its saturated
What type of dependence does Vo have on [S]? Meaning as [S] increase, what happens toVo?
Zero order, no change to Vo
What is the bottom linear line represent of the MandM eq in terms of dependency?meaning?
Represents the 1st order dependency of Vo on [S] meaning as [s} increase the Vo increases
What does the equation (Vmax/Km)xS represent in the graph and what conditions are these?
Corresponds to the First bottom Linear line when [S] <<< Km (Shows 1st order of dependence)
What does the equation Vo=Vmax represent on the M and M graoh?
Represents the horizontal linear line for Vmax
What does a low Km mean?
Binds substrate more tightly to the enzyme making it prcocess less substrates
What does a high Km mean?
binds the substrate more losely to the enzyme so process more substrates/metabolites
What values can enzymes change in response to some bio happenings?
Km valuse because in the same rxn you can have different Kms
Where is hexokinase and glucokinase found?
Hexo is found everywhere BUT the liver and gluco is found in the LIver
What type of Km values do hexokinase and glucokinase have?
HExo=Low Km (Proocess less and "sucks up" ess glucose) Gluco=High KM (sucks up excess glucose
What does Kcat/Km give an indicator for?
how fast the product is formed from the E+S free in solution
What does Kcat/Km help determine?
Which substrate is better for an enzyme if there is more than one substrate
Types of bonds found in Reversible and Irreversible inhibtition
Irriversible DOES have covalent bonds btween the E and the inhibitor when eversible doesnt
What types of inhibitor is 5 Florouricil? and what does it inhibit?
Irreversible ihinibitor of thymidylate synthease
What type of inhibitor is Methlytrexate and what does it inhibit?
Reversible inhibitor of dihydrolate reductase
What do Methlytrexate and 5 Florouricil do?
interphere with DNA synthesis in papidly growin cells including hair and stomach cells
Where do competive inhibitor bind?
at the active site BUT are NOT processed- just block the actie ste
What changes and stays the same as the amount of competive inhibitor is increase?
(1/vmax) is constant and (-1/Km) becaome less negative/increases [Kapparent increases)
Why does V max remain constant?
Because if there is a ridiculous high amt of substrate, then the competive inhibitor is going to basically not beat out all the substrates for the active site- therefore the inhibitor is diluted out
Why does K Apparent Increase?
An inhibitor slows or stops the enzymative process therefore Vo is slow and it takes more [S] to get to 1/s of the Vmax- As more and more inhibiotr is added- the steeper the line gets thereforeless substrate is needed to get to half of vmax (look at graph)
What is an example of a Competive inhibitor? Why?
Malanate competetively inhibits Succinate because of its similar structioe
What does K cat tell you?
how fast an enzyme can go when its compelety saturated with substrate aka its the Vo value of Vmax's point on the graph
What part of the equilibrium equation is Kcat enzymes?
In the **ES**--> E + P part bc Kcat measures how fast ES complex breaks down (aka turn over)
What units are associated with Kcat? what do these tell you
min-1 or sec -1- tells you the number of moles of S converted to P/ unity of time divided by the moles of E
What does it mean when ACh estrace has a high Kcat?
it breaks down ACh very fast and has a high turn over
How are trp from peptide chain and Indole similar in the way they look?
The TRP of the peptide chain has a R group that IS indole
What is the cause when indole binds in chymotrypsins active site?
The peptide can't bind and be hydrolyzed (REMEMBER: chymotrypsin cleaves at Trp )
Wat is the third example of competitive inhibitor? (Make lots of money)
Zocor and Lipitor (-statin drugs) for the inhibition of HMG-COa
How do non competitive inhibitors differ from competitive?
DO NOT bind at active site or resemle substrate
Frequency of non competitive inhibitors in drugs?
Not common- don't know where going to bind so hard to design
Describ the line weaver burke plot for Non Competitive inhibitors?
Km is Constant, (1/vmax) increases and Vmax decreases
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