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Lab 12: Microbial control
Terms in this set (23)
= How a microbial control agent works
mode of action
= Reducing the number of microbes on an inanimate surface
= Reducing the number of microbes on an animate surface
= Elimination of all microbial life, including endospores
What are the 2 types of antimicrobial drugs?
- Broad spectrum
- Narrow spectrum
= property of antimicrobial drugs that allows them to be effective against target cells while causing no harm to host cells.
= drugs that affect a variety of bacterial types
= drugs that affect 1 or few bacteria types
What is the mode of action of UV light?
to create thymine dimers in DNA, preventing transcription and replication
Does UV light kill endospores?
Which drugs function to disrupt cell wall synthesis (peptidoglycan)?
Which drugs function to disrupt protein synthesis (70s ribosomes)?
Which drugs function to disrupt DNA replication (DNA gyrase)?
Which drugs function to disrupt folic acid synthesis?
= Filter paper disks that contain antimicrobial placed on a freshly swabbed Mueller-Hinton agar plate
Kirby Bauer Disk
Why is UV light not used for sterilization?
UV light is not effective in killing endospores.
How does exposure time affect the efficacy of UV light?
The longer the exposure to UV, the more effective it is in controlling microbial growth.
What is the difference between antisepsis and disinfection?
Antisepsis is used on living tissue, disinfection is used on inanimate surfaces.
What is similar between antisepsis and disinfection?
Antisepsis and disinfection both reduce the number of microorganisms on surfaces.
What is measured to assess the Kirby Bauer disk diffusion assay?
The diameter of the zone of inhibition is measured.
What medium is used in the Kirby Bauer disk diffusion assay?
Mueller-Hinton agar is used for the disk diffusion assay.
What would you hypothesize to explain why there may be differences in the usefulness of antibiotics on different species of bacteria?
Inherent differences in the PHYSIOLOGY and STRUCTURE of bacteria lead to different reactions to antibiotics. For example, penicillin is not effective against Gram-negative bacteria.
How does UV light affect bacteria?
UV light, a type of non-ionizing radiation, produces thymine dimers in DNA. Thymine dimers alter the shape of the DNA molecule and disrupt the functions of DNA.
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