Some sporozoans can do asexual via Schizogony, and/or asexual via Sporogoy
What is the protective/infectious form of protozoa?
CYST! protects from env and helps in transmission
Overall, how do cysts get back to environment?
we usually ingest in cyst form, people poop them out
What are the 5 protozoa we are going to talk about here?
Entamoeba histolytica, Giardia lamblia, Cyclospora, Isospora belli, and Cryptosporidium parvum
What disease does Entamoeba histolytica cause?
What is the infectious form?
How is it transmitted?
trophozoites will not survive in the env. or gastric acidity, so they are not infectious
Pathology? Where will it multiply? Is it invasive?
Cysts hatch in GI goes to either colon or circulatory multiplies in colon; INVASIVE
What is the claim to fame?
only ameoba that causes disease
other non-pathogenic species confused for E. histolytica. E. dispar often responsible for asymptomatic infections
What are the secondary effects**** of the pathogenesis of Entamoeba histolytica?
lysis RBC's and forms brain and liver abcesses
All symptomatic pts have will present with visible or microscopic blood in their stool (dysntary)
NO fever! this is a way you can distingiush ameobic dysentary from other dysenteries
Symptomatic vs. Asymptomatic? and passing on disease?
Symptomatics have the Tropho-fom but have a harder time passing the disease on
Asymptomatics have cyst form but pass it on easily
lacks a sexual stage infectious cyst form and an invasive trophozoite form
cyst can survive in env for weeks, enters humans, motile trophs are released from cysts in the small intestine and go to large: then can invade host tissues (symptomatic) or live in the lumen of LG Intestine without invasion (asymptomatic) or encyst and be shed from host
What is the shape of Entamoeba histolytica penetration lesion into the intestinal wall?
When is the only time we see fever with Entamoeba histolytica?
When they get through intestine, into circ., then into liver and make abscesses get fever and abdominal pain, and WBC increased
Stool sample, observational see cysts or trophozoites biobsy and serology (extra-intestinal) reacting to antigens of the ameoba
When do we see watery stool: with more trophs or more cysts?
more trophs = watery
more cysts = formed
What are the 2 forms of Giardia lamblia?
1: flagellated looks like a sting ray w/ 2 eyes
2: Cyst normal cyst form
Path? Where will they settle? How is this done?
Ingested as cyst excyst in duodenum multiplies by binary fission on mucosa of duodenum--> NO INVASION Adheres to mucosa in intestines using a suction disk on the front of their body --> in feces
Age group of target? Why?
VERY young (b/c kids can be gross); 25-40 y. olds b/c of the recreational sports/being outside a lot
peaks in summer to late fall can be animal reservoirs
symptomatic: pass trophozoites asymptomatic: pass more cysts
What harbors Giardia lamblia a LOT?
Where can outbreaks (locations)?
Day care centers and workers there; recreation spots
Sx? (asymptomatic vs. symptomatic?)
If symptomatic, then have trophs, so get foul smelling, greasy, floating stool with NO BLOOD (b/c no invasion of mucosa, just attachment), cramping, bloating, gas, NO fever
If asymptomatic, you have cysts and spread Giardia to all your friends
How long does disease last?
Acute: weeks Chronic: months to years malnutrition due to malabsorption
observe cysts in formed stool observe trophs in diarrheal stools
water treatment/filtration when hiking, etc.
Giardia Troph and cyst: what do they look like?
Troph: teardrop shaped, two nuclei (eyes) four pairs of flagella, claw-shaped median bodies, ventral disk, tumbling swimming motions
Cyst: oval and smooth walled prominent transvers claw-shaped median bodies and longitudinal axostyle
What is the only other of 5 flagelletes that causes disease?
dientomoeba fragilis and most are asymptomatic if symps: ab pain, flatulence, diarrhea, and weight loss
no cyst stage
What is the claim to fame for Balantidium Coli?
only ciliated pathogen associated with disease in humans
fecal oral from pigs
Sx generally? Can cause?
Asymptomatic; alternating bouts of diarrhea and constipation CHRONICALLY
The acute path is?
INVASIVE so can have blood and pus in stool simialar to amebic dystentary
Transmission of Cryptosporidium parvum?
What do you ingest, in particular?
Type of disease caused by Cryptosporidium parvum?
mature oocyst with sporozites gets ingested, then the sporozoans attach to intestinal epithelium and mature, then sexual forms develop and produce fertilized oocyst, mature oocyst in feces
asexual reproduction in small intestine, merozites are relased into the lumen and can infect new intestinal cells perpetuating asexual cycle
What type of reproduction for Cryptosporidium parvum?
What is another source for Cryptosporidium parvum?
very youn and 25-40 yrs
Profuse watery diarrhea with cramps, fatigue; NO BLOOD B/C not invasive
Where can we see Cryptosporidium parvum?
Water, public pools contaminated drinking water
oocyte in stool
What is the Tx?
mainly self limiting
proper sanitization for drinking water
Who is also at risk here?
immunocompromised, AIDS (15-25% of these pts are infected) can be fatal for AIDS patients, so immunity is important here both Ab and cell mediated
fever, diarrhea, weight loss
immature oocyst with sporocyst is injested, mature, sporozoites divide and mature in small intestine of mucosa, sexual forms develop and produce fertilized cyst, becomes oocyst with zygote in feces
Who is most seriously effected?
AIDS PTs: second only to cryptosoridium for causing diarrhea in AIDS pts.
get Watery diarrhea, malabsorption, wt. loss, death
unsporulated oocyte shed from infected folks oocyst sporulation in env --> fecal to oral transmission oocyte get ingested from contaminated env
PROFUSE WATERY DIARRHEA, may have RASPBERRY SEEDS, cramp, fatigue, wt. loss
Disease in AIDS pts more serious
Stool sample with FLOURESCING oocytes under UV!!!!!
intestinal disease or disseminate throughout the body, microsporidiosis
Type of parasite?
obligate intracellular parasite
What we know about disease if from?
increased occurance in AIDS pts
Chronic debilitating diarrhea, anorexia, weight loss, 10-20 stools a day
Extra intestinal Sx?
keratoconjunctivitis is an early indicator of dissemination
symps depend on organ infected
Which has diarrhea that is foul smelling, greasy, and floating?