# LS23L Final

The Sanger method does NOT use which of the following:

Select one:
a. RNA as the template to be sequenced
b. dNTPs as the building block of the newly synthesized strands
c. a sequencing primer for annealing to the template
d. ddNTPs as the terminator of the newly synthesized strands
e. DNA polymerase to catalyze the reaction
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The Sanger method does NOT use which of the following:

Select one:
a. RNA as the template to be sequenced
b. dNTPs as the building block of the newly synthesized strands
c. a sequencing primer for annealing to the template
d. ddNTPs as the terminator of the newly synthesized strands
e. DNA polymerase to catalyze the reaction
An ectotherm is:

Select one:
a. An animal that continually radiates heat to its environment
b. None of these
c. All of these
d. An animal that is capable of generating its own body heat therefore its body temperature stays constant over a wide range of ambient temperature.
e. An animal that obtains its body heat from the environment therefore its body temperature varies with outside temperature
Does the mitochondrial DNA sequence amplified by PCR in the DNA Isolation lab code for functional proteins?

Select one:
a. No, the amplified sequence is located in the control region, and therefore does not control the coding of functional proteins.
b. No, mitochondrial DNA sequences never code for functional proteins.
c. No, only RNA sequences and nuclear DNA sequences code for functional proteins.
d. Yes, all mitochondrial DNA sequences code for functional proteins.
e. Yes, the amplified sequence is located in the control region, and therefore controls the coding of functional proteins.
All of the following is true about agarose gels EXCEPT:

Select one:
a. Agarose gel electrophoresis can be used for the separation of DNA fragments.
b. Agarose gel electrophoresis has a higher resolution than SDS-PAGE.
c. Agarose is safe to handle, whereas acrylamide is a neurotoxin.
d. Conventionally, agarose gel electrophoresis is set up horizontally.
e. The pores in agarose gels are bigger than those in polyacrylamide gels.
In the Gel Electrophoresis lab, why did we not add SDS and beta mercaptoethanol to the agarose gel before loading our PCR product?

Select one:
a. The PCR product is already linear and has a negative charge associated with it.
b. The agarose gel was run horizontally instead of vertically.
c. The PCR product is so small that SDS and beta mercaptoethanol are not needed.
d. The GelRed acted as a reducing agent and gave the PCR product a negative charge.
e. The agarose gel is non-toxic, so we do not need SDS and beta mercaptoethanol.
Which of the following is an INCORRECT statement regarding the differences between a dissecting microscope and a compound microscope?

Select one:
a. Dissecting microscopes do not have as high a magnification as compound microscopes.
b. The magnification of a compound microscope is discrete, while the magnification of a dissecting microscope is continuous.
c. A compound microscope displays a mirror image of the object, while a dissecting microscope displays an image in the same orientation of the object.
d. Light shines on the sample for a dissecting microscope, while light penetrates a sample for a compound microscope.
e. The compound microscope is used for gross morphological examination, while the dissecting microscope is used to examine cellular structure.