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20 terms

micro lab 2

STUDY
PLAY
inoculation
the introduction of microorganism to culture media
inoculum
sample of micro organisms
sterilization
the eradication of all organisms in or on an object
bacti-cinerator
ceramic flame-free sterilizers used to sterilize loops and needles
autoclave
device that uses steam heat under pressure to sterilize chemicals and objects that can tolerate moist heat
agar
gel like polysaccharide isolated from red algae and used as a thickening agent for culture media
culture
act of growing microorganisms of the microorganisms that are cultivated
culture media
provides nutrients for culturing microorganisms
3 types of media used
broths, agar slants and agar deeps.
nonpathogenic
do not cause disease
pathogens
microbes capable of causing disease
opportunistic pathogens
microbes that cause disease when the immune system is suppressed or when introduced into an unusual area of the body.
reservoir
any area where a microbe resides that serve as a potential source of infection
commensals
microbes that benefit from living in/on our bodies without significantly affecting us. may become opportunistic pathogens.
mutualistic
microbes that benefit from living on/in our bodies and provide some benefit to us as well. May become opportunistic pathogens
stains
solutions consisting of a colored molecule dissolved in a solvent, such as ethanol
chromogen
a colored molecule
basic stain
positively charged stain that binds to negative electrical charges on the surface of bacteria
acidic stain
negatively charged stain that is repelled by bacteria and stains the slide
heat fixing
procedure that kills bacteria, adhere them ti the slide, and coagulates their proteins