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Differential media micro lab final 3

Terms in this set (32)

What makes media differential
Indicators
If bacterial organism has no growth on phenyl ethyl alcohol agar what organism is it
Gram negative organism
Alpha hemolysis will result in the ________agar
In complete cleaning or greening of
Coil forms are___________bacterial that ferment__________
Gram t, lactose
Hektoen enteric agar primarily designed to differentiate between
Salmonella and shigella
Media contains indicate is to expose differentiate between organisms is called
Differentiate
Mannitol Salt Agar (MSA) turns __________ color when ferment is occurring
Yellow
_______________is inhibiter in Maconkey agar while____________is incater
Crystal violets and blue salts, neutralized
TSA classified as
Neither selective nor differential
Indicator in phenyl ethyl alcohol agar (PEA) is
No indicator, PEA is non differential
Which agar will cause Ecoli colonies to have green metallic
Eosin meylyn blue agar
In MSA agar, mannitol is the
Substrate
Streak unknown on heckten (HE) incubate 48 hours often black colonies on blue agar what occurred?
Sulfer veeluetion (organism is gram negative)
______________is the initial source of sulfer w/in hektoen enteric agar (HE)
Sodium thiosulfate
After incubation an unknown organism on macconeky agar the media turns yellow, what happened?
A. Fermentation of mannitol
B. ". ". Lactose
C. A plt change to an acidic environment
D. Non
Which is not acharastetic of blood agar?
A. It is differential
B. It is differential for lucynsis type
C. Beet hemolysis will show a complete lysyming of
D. Gamma hemolysis will show growth, no lysing of blood
E. All are correct
The streaking is isolation method is___________
A form of dilution used to separate mixed cultures
You streak an unknown organism on embagar and let it incubate for 48 hours after incubation you see purple colonies what is you're results
Tg and F+
_____________encourages the growth of most culturable bacteria
TSA
Lactose fermentation on HE is going to turn media what color
Orange
__________________is the inhibitor in MSA
Salt NaCl 7.5
Blood agar contains 5 percent _____________blood
Sheep
_____________are what makes a medium selective
Indicators
Positive fermentation on macconkey agar is going to turn the agar pink
...
Coil forms are used in __________quality testing
Water
_________________are the nutrients that bacteria use to grow in the media
Substrates
______________heymolysis in the complete lysis of blood cells
Beta
_________ coliforms often colonize the large intestine
Fecal
_______________is the indicator for lactose fermentation in HE
Bromotymol blue
What is the complete reaction that causes blaek colonies on HE
Sodium thiosulfate (red bacteria)—————> hydrogen sulfice =fiend ammonium citrate (blaek colonies)
What ingredients in eosin methylene blue agar make it selective
Eosiny meythyln blue selective for gramming
what is the main rule of the streaking to isolation method
CFU————->colony forming unites
Isolating/looking at exact colonies of bacteria
One bacterial cell=one colony
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