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ABO Grouping (Principle)
detection in the patients serum with known commercial anti-sera
reverse-patients plasma with known red cells
is used to determine blood group and what type of blood or blood components can be received safely.
red cells possessing the D-antigen agglutinate in the agglutinate in the presence of anti-d
Principle DAt (purpose)
to detects in-vivo sensitization of RBCs with IgG and complement components.
Clinical Conditions DAT
HDN, Hemolytic Tranfusion reaction, Autoimmune and drug induced.
used to determine if RBCs have been sensitized with complement components C3b or C3d.
used to determine if RBCs have been sensitized with IgG antibody
used to determine if RBCs have been sensitized with immunoglobulin, gamma globulin for antibody or beta globulin for complement (C3b or C3d) or both.
IgG Coombs Check Cells
used to verify negative results of antigloubin tests.
Complement Check Cells
used to confirm reactivity of the anti-C3 component of AHG.
Antibody Screen Cells
used for the detection of unexpected antibodies for pre-transfusion and donor testing.
LISS- Low Ionic Strength Solution
low salt media used to decrease the ionic strength of a reaction medium which reduces the zeta potential and therefore allows antibodies to react more efficiently with RBC membrane antigens
additive used to enhance sensitivity in the detection of unexpected blood group antibodies.
primarily used as a control for group AB type Rh positive patients as a means to rule out autoagglutination.
AHG Reagents- Anti-Human Globulin
used when RBCs become coated with antibody or complement or both but do not agglutinate in regular testing
The direct antiglobulin test
test shows whether or not red cells have been sensitized in vivo and is done after washing the cells of unbound serum proteins
patient has a positive antibody screening test or has a previous record of clinically significant antibody
patient has a negative antibody screening test or has a no record of clinically significant antibody
the testing of the patients serum with the donor's red cells, to confirm abo compatability
to detect clinically significant anti-bodies as a part of pretransfusion.
The indirect antiglobulin test
The indirect antiglobulin test (IAT) looks for circulating antibodies in the blood directed against red blood cells (RBCs).