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Week 15- genetics
Terms in this set (53)
DNA binding proteins
similarities between prokaryotes and eukaryotes
promoters, chromatic structure, nuclear membrane
Differences between Eukaryotes and prokaryotes: what do eukaryotes have that prokaryotes do not?
Gene expression is "off" unless specifically turned on by the binding of specific regulator proteins
Which of the following statements best describes the "ground state" for expression of genes in eukaryotic cells?
Transcriptional gene regulation in Eukaryotes: processing..
miRNA and siRNA
Transcriptional gene regulation in Eukaryotes: degradation..
target mRNA, translation, target mRNA
Transcriptional gene regulation in Eukaryotes: miRNA degrades __________________ and inhibits ____________________ of _____________________.
target mRNA, siRNA
Transcriptional gene regulation in Eukaryotes: siRNA degrades ____________________ and uses ________________ to target genes for methylation/silencing.
modification of histone proteins
Methylation of DNA
Chromatin structure represses...
gene switched 'on', active chromatin, unmethylated cytosines, acetylated histones
gene switched 'off', silent chromatin, methylated cytosines, deacetylated histones
Expression is 1000 fold higher in the presence of...
Transcriptional activator proteins bind to _________________ in yeast
gene activation, silent
UAS elements are essential for _________________________ if deleted genes are _______________ even with galactose.
Transcriptional activator proteins are....
transcriptional activator proteins may be activated by...
transcriptional activator proteins recruit the...
effector molecule-->intermediate binding protein-->repressor-->transcription factor
steps in eukaryotic transcription
Yeast, Gal 4 system: intermed. binding protein=?
Yeast, Gal 4 system: effector=?
Yeast, Gal 4 system: repressor=?
Yeast, Gal 4 system: transcription factor=?
Auxin regulation: effector=?
Auxin regulation: intermed. binding protein=?
Auxin regulation: repressor=?
inhibitor of ARF
Auxin regulation: transcription factor=?
auxin response factor
low auxin= gene expression...
high auxin= gene expression...
Cis-acting regulatory sequence in DNA that acts (from a distance) to promote the expression of specific eukaryotic genes
gene that is normally off, turned on during viral infection
largest transcription family
one body part replaced by another
Triple mutant in Drosophila caused by what gene?
more than 1 pair of wings (Ultrabithorax)
Ubx genes causes flies to have...
homeotic mutant of Drosophila where antennas are transformed into legs, caused by what mutant?
Hox genes (homeotic genes)
genes that regulate position of anatomical features in organisms; regulates identity of body parts
identity of segments
Primary role of Hox genes during normal drosophila development is to determine the...
Bithorax complex: ________ is responsible for wing development
Abd-A (abdominal A)
Bithorax complex: ________ is responsible for gonad development
Abd-B (abdominal B)
Bithorax complex: ________ is responsible for pigmentation differences in males and females
Antennapedia complex: _________ responsible for developing head and mid-gut
sex combs reduced
Antennapedia complex: Scr stands for...
Antennapedia complex: _________ responsible for developing labial and palps
Antennapedia complex: _______________ responsible for deformed adult maxillary palp
Antennapedia complex: _______________ responsible for adult leg
Expressed in developing hindwing, but absent in developing forewine
Ubx(Ultrabithorax) is a Drosophila homeotic gene that normally has two key functions: promote hindwing development and repress forewing development. The Ubx protein is a transcription factor (and activates the transcription of certain genes). Knowing this, this gene would likely be:
promote hindwing development and repress forewing development
A nonlethal loss-of-function allele of Ubx might be expected to transform the __________________ into a _____________________.
Order of __________ genes parallels the order of body parts in which they are expressed
genotype of the mother
The distinctive feature of maternal effect genes is that the phenotype of the embryo depends solely on the..
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