20 terms

Compound Light Microscope Parts Definitions

The definitions of the parts of the compound light microscope.
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Body Tube
cylinder with the eye piece at one end and the nose piece at the other
Nosepiece
revolving structure at the bottom of the body tube containing the objective lenses
Stage
flat platform with a hole in the center to allow light through. it holds the specimen
Diaphragm
The diaphragm controls the amount of light that passes through the stage. as the magnification increases the amount of light necessary to produce a clear image also increases.
ocular lens (eyepiece)
located on the top of the body tube. allows light to pass through and increases the image usually 10x to 15 x
Arm
The arms supports the body tube and is used for carrying the microscope.
Coarse Adjustment
The coarse adjustment knob is used to focus the low power only. gives a rough picture of the specimen
Fine Adjustment
used for sharp final focusing. it focuses to the indivduals eye. the clearer the picture the higher the microscopes resolving power
clips
hold the slide to the stage
resolving power
the ability to distinguish between to points
objective lens
located on the revolving nose piece and have a variety of magnifyingpowers.
total magnification of compound microscope
multiply magnification of the ocular lens and the objective lens.
dissecting microscope
steriomicroscope. low magnification potential.
compound light microscope
uses a series of lenses to produce an enlarged image
phase contrast microscope
similar to a compound microscope with exception that the light strikes the specimen at an angle allowing for three dimensions to be seen instead of only two.
electron microscope
uses beams of electrons to make an image. it magnifies about 200,000 x. can only study dead organisms.
microdissection instruments
used with a comound microscope. cell organelles can be added removed or transferred. can not be seen with naked eye.
stains and dyes
make certain areas of a specimen easier to view. all stains with exception of vital stsins kill the specimen.
ultracentrifuge
spins test tubes at about 50,000 rpms seperating the cell structures according to their density. large amounts of certain organelles can be gathered at one time.
chromatography
used to seperate and analyzing mixtures of chemical substances. seperated according to their densities.
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