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41 terms

transport from ER through Golgi apparatus

STUDY
PLAY
carbohydrate synthesis
golgi apparatus is major site of ____
pectin and hemicellulose
synthesis of ____ of plant cell wall
glycosaminoglycans
synthesis of _____ of the extracellular matrix in animals cells
sorting and dispatching
__station for products of the ER
oligosaccharide
attaches ____ side chains to proteins and lipids from ER for targeting into the transport vesicles destined for specific destinations
COPII
proteins leave ER in ___ - coated transport vesicles
ER exit sites
COPII-coated vesicles bud from specialized regions of the ER called __
exit signals
Proteins that enter vesicles that leave the ER is a selective process mediated by ____
ER resident proteins
some _____ "leak" out of the ER and go to the golgi
secretory proteins
___ that are made in high concentrations may leave the ER without exit signals
ER to golgi
retention of incompletely assembled antibody molecules in the ER
BiP
The chaperone protein ___ binds to incompletely assembled antibodies
chaperone proteins
___ cover up exit signals on the incomplete molecules
homotypic membrane fusion
fusion of membranes from same compartment
step 1 homotypic membrane fusion
NSF pries apart identical pairs of v-SNARES and t-SNARES in both membranes
step 2 homotypic membrane fusion
matching v-SNARES and t-SNARES from adjacent identical membranes interact
step 3 homotypic membrane fusion
membranes from both vesicles fuse to form vesicular tubular cluster
homotypic membrane fusion
vesicular tubular clusters
____ can release COPI-coated vesicles which return resident proteins to the ER
ER retrieval signals
__ from escaped resident ER proteins binds to COPI coats for return to the ER
vesicular tubular clusters
KDEL sequence
__ signals a soluble ER resident protein
KDEL receptor
__mediates the ER resident protein to become vesiculated and COPI coated
KDEL
Retrieval pathway to the ER uses sorting signals
cis-face
entry face, is closest to the ER associated with cis golgi network
trans face
exit face, is closest to plasma membrane associate with trans golgi network
golgi apparatus
modifications to oligosaccharide
1. Man = Mannose 2. GlcNAc= N-acetylglucosamine 3. Gal=galactose 4. NANA=N-acetylneuraminic acid
oligosaccharide processing in golgi
modified oligo's
vesicular transport model
vesicular transport model
golgi apparatus is a static structure while the molecule in transit move through the cisternae sequentially via transport vesicles
cisternal maturation model
cisternal maturation model
golgi is a dynamic structure in which the cisternae move and mature
cis golgi network
phosphorylation of oligosaccharides on lysomal proteins
cis cisternae
removal of Mannose
medial cisternae
removal of mannose and addition of GlcNAc
trans cisternae
addition of Gal and addition of NANA
trans golgi network
sulfation of tyrosine and carbohydrates