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Terms in this set (39)
Which of the following is the definition of biotechnology?
The use of living systems to benefit humankind
Which of the following is the definition of genetic engineering ?
The alteration of DNA in a cell
The first set of genes to be introduced into e.coli from which of the following?
Which of the following is a palindrome sequence that could be recognized by a restriction enzyme
5′ - GAATTC - 3′
3′ - CTTAAG - 5′
restriction enzymes (endonucleases) are produced by bacteria as a means of protection against which infectious agent ?
Which of the following enzymes rejoins two sugar-phosphate backbone of dna ?
Genes to be cloned into a plasmid are typically inserted in which location?
Which genus of bacterium is naturally competent ?
Which of the following regarding puc19 and blue/white screening is not true?
The gene of interest being cloned disrupts the lacI gene.
You are in the process of cloning a gene of interest into pUC19 using blue/white screening. You complete your cloning and transform E. coli with your cloned plasmid. Youplate your transformation ontwo media. One medium agar plate contains X-gal only. The second medium agar plate contains X-gal and ampicillin (an antibiotic), in the hope of using blue/white screening. You examine your plates and see a mixture of blue and white colonies. Which of the following colonies should be picked?
The white colonies that are resistant to ampicillin
Which plasmid encodes the genes for its own conjugation?
An F plasmid
Which of the following methods can be used to transfect protoplast plant cells?
All these options can be used ( gene gun, electroporation, agrobacterium tumefaciens)
Which virus is typically used as mechanism to transfect eukaryotic cells?
For gel electrophoresis, the matrix used for separation of DNA fragments is ________,whereas the matrix used for separation of proteins is________.
Which charge does a molecule of DNA have?
A ________ blot is used to identify specific sequences of DNA on a membrane, whereas a ________ blot is used to identify specific sequences of RNA on a membrane.
A two-dimensional PAGE separates molecules according to which two properties?
charge at various pHs and size
For protein electrophoresis to function, which of the following can be added to the protein to impart a uniform negative charge?
sodium dodecyl sulfate
. Which technique can be used to amplify DNA?
Which of the following are the three steps (in order) of PCR?
denaturation, annealing, extension
Which of the following correctly explains why primers are used in PCR?
DNA replication requires a free 3' hydroxyl group
The Sanger method/chain termination reaction relies upon using which unique nucleotide?
a nucleotide that is missing a hydroxyl group
Which of the following isa next-generation sequencing technique in which fragmented DNA has DNA adapters attached, is amplified by PCR, is attached to a bead, and then is placed into a well with sequencing reagents.The flash of light produced by the release of pyrophosphate on addition of a nucleotide is monitored
The study of all the DNA of an entire microbial community is known as which of the following?
In RNAi technology, the dsRNA molecule first combines with which endonuclease that then cleaves it into smaller fragments?
Which of the followingis/are completely complementary to the target mRNA in RNAi technology?
Which disease has been effectively controlled by gene therapy?
Which agency does not oversee gene therapy?
Genomics would not be an effective method to detect which type of pathogen?
DNA that has been cut with a restriction enzyme, resulting in sticky ends, can be more easily ligated than DNA that has been cut with a restriction enzyme resulting in blunt ends.
For cloning purposes using transduction, lytic bacteriophages are used
Lambda (l) bacteriophages can be used to make genomic libraries
The uptake of a plasmid by a prokaryotic cell is known as transfection.
DNA probes can be used to locate any gene within a cell.
Blue/white screening works on the basis of disrupting the lacZ gene in cloning.
Reverse transcriptase-PCR amplifies molecules of RNA
cDNA is made by reverse transcriptase, which is found in all viruses
Due to concerns aboutgene transfer, genetically engineered microbes cannot be used to manufacture vaccines
RNAi is a natural process used by cells as a means of gene regulation
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