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Biochem Unit 4 Material

Terms in this set (24)

What amino acid is covalently linked to lipoic acid in E2 of the pyruvate dehydrogenase complex?
Lys
Which of the following is an anaplerotic reaction that is often used in the cell?
conversion of pyruvate to oxaloacetate
Which enzyme catalyzes a reaction most similar to that catalyzed by α-ketoglutarate dehydrogenase?
pyruvate dehydrogenase
Which of the following is an intermediate between citrate and isocitrate in the citric acid cycle?
cis-aconitate
Where is succinate dehydrogenase located?
Group of answer choices
mitochondrial inner membrane
Which of the following enzymes catalyzes a substrate-level phosphorylation?
succinyl-CoA synthetase
A 0.10 M imidazole solution has a pH of 6.5. To the nearest hundredth of a unit, what fraction of the molecules are in the neutral (imidazole) form? (The pKa of the imidazolium ion is 6.00.)
10^(pH-pKa)........ (ans)/(ans +1)
.76
If 4 molecules of a particular protein were present in an E. coli cell, what would be the nanomolar concentration of this protein? Assume an E. coli cell to be a cylinder 2 micrometers long and 1.09 micrometer in diameter. Report your answer to the nearest tenth of a unit. Please note that the units are understood to be nanomolar (or nM), but just enter the numerical answer and not the units. I do not want people to miss this question because one person entered "nanomolar" and another person entered "nM".
(molecules/avagadros number)/(volume in liters)
Multiply by 10^9 for answer in nm
3.6
What is the pH (to the nearest hundredths) of a 0.11 M solution that was made with the sodium salt of a carboxyl-containing organic compound? The pKa of the acidic form of this compound is 4.55.
convert to Kb, multiply by concentration, take square root and then -log that answer
14-ans
=8.8
A compound has two amino/aminium groups and one carboxyl/carboxylate group. The respective pKas are 8.5, 9.5, and 2.3. To the nearest tenth of a unit, what is the isoelectric point of this compound?
Basic so take two highest pka values
=9
Determine ΔG°' for the redox reaction in which FADH2 is oxidized to FAD and oxygen is reduced to water (using the reduction potential values given below). Next, decide how many ATPs could be synthesized from the available free energy that the above redox reaction would liberate (assuming that it costs 10.9 kcal/mol to synthesize ATP in cells rather than the 7.3 kcal/mol it costs using biochemical standard state conditions). Finally, determine the efficiency of ATP synthesis using the above calculations and the known stoichiometry: one FADH2 produces 1.5 ATP. What is this efficiency? Enter your answer as a percent to the nearest ones.
43
What is the energy of a mol of photons (an einstein of photons) that have a wavelength of 671 nm? Recall that Planck's constant (h) is 6.626 x 10-34 J⋅s and the speed of light (c) is 3.0 x 108 m/s. Enter your answer in terms of kcal/mol to the nearest tenth.
E=hc/(lambda)
multiply this by avagadros number
divide by 4184
=42.6
What will be the likely effect of adding oxygen to a culture of cells that had been growing anaerobically?
The rate of glucose consumption will likely increase.
Imagine that the CO2 used in the rubisco reaction was labeled with 14C. Which of the following correctly describes the location(s) of labeled carbons in the 3-phosphoglycerate (3PG) molecules that are produced in the rubisco reaction? Assume the reaction only uses CO2 (and not O2), and that the 3PG are removed as they are formed (to make the reaction unidirectional).
The third (i.e., phosphomethyl) carbon of all 3PG produced
Imagine an in vitro experiment that contains unlabeled glyceraldehyde 3-phosphate (GAP) and sedoheptulose 7-phosphate that is labeled with 14C at C3. Imagine further that the scientist conducting this experiment includes transketolase, phosphopentose epimerase, and phosphopentose isomerase and all necessary cofactors, metal ions, etc. Where will the label appear in the ribulose 5-phosphate molecules (Ru 5P) that ultimately are produced?
Group of answer choices
C1 of all Ru 5p thus produced
In the pathway that plants use to synthesize starch, when glucose 1-phosphate reacts with ATP, what are the products of the reaction?
ADP-glucose and inorganic pyrophosphate
If pyruvate and valine undergo a transamination (i.e., aminotransferase) reaction, what will be the product(s) of the reaction. Select all that apply.
3-methyl-2-oxobutanoate
alanine
The amino acid serine is synthesized from three steps starting with a glycolytic intermediate. What is the glycolytic intermediate that gives rise to serine after three steps?
3PG
The important, short-range signaling molecule nitric oxide is derived from which of the following amino acids?
Group of answer choices
Arg
In the urea cycle, the formation of carbamoyl phosphate predominantly takes place in the
mitochondria of hepatic (liver) cells
In the urea cycle, what amino acid donates an amino group directly into the cycle? (Assume all choices are the L-form of the amino acid.)
Aspartate
The β-cells of pancreatic islets of Langerhans produce __________ in response to __________ glucose levels.
Insulin; high
The α-cells of pancreatic islets of Langerhans produce __________ in response to __________ glucose levels.
glucagon; low
What is the name of the German biochemist who is considered by many to be the preeminent biochemist of the first half of the 20th century? (Hint: He purified and studied many of the enzymes of glycolysis and he discovered a link between glycolysis and cancer.)
Group of answer choices
Otto Warburg
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