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The Sanger method of DNA sequencing involves which of the following:
The use of dideoxyribonucleotides that function as chain terminators
vitro DNA synthesis reaction
Two closely related strains of Streptococcus, one requiring tryptophan and alanine & the other requiring leucine and arginine, were placed in the separate arms of a U-tube containing medium supplemented with DNAse. After several hours of cultivation, samples were taken from one arm and plated on minimal agar lacking any of these four growth supplements. Several colonies grew on the minimal media. Which of the foll
The haploid human genome contains approximately ______ base pairs and codes for approximately _______ genes
Fill in the blanks. In the trp operon of E. coli, _________________ functions as the effector molecule by acting as a(n)___________________.
tryptophan -- co-repressor
An F plasmid integrates into the bacterial genome most often at
In eukaryotes, an enhancer is
A cis -acting sequence recognized by transcription factors, whose function in activation of gene expression is largely independent of its location and orientation relative to the transcription start site.
Typically, mutations that convert a proto-oncogene into an oncogene ___________________
are gain-of-function and dominant.
An oncogenic form of Ras would ________.
have decreased GTPase activity
Checkpoint proteins are encoded by ________.
Genomic imprinting is a result of ________.
In mammals, most steroid hormones regulate gene expression by:
binding to a cytoplasmic receptor, which then migrates to the nucleus and functions as a transcription factor.
Bisulfite sequencing is used to identify/determine which of the following
Patterns of cytosine methylation.
An E. coli cell has the genotype I-, O+, Z+, Y+, A-. Which of the following statements regarding expression of the structural genes of the lac operon is TRUE
The structural genes will be constitutively expressed
. If the E. coli strain in Problem 15 above is grown in medium containing glucose, the structural genes will _____________
not be highly expressed regardless of whether lactose is present or absent
The lacIs mutation prevents the lac repressor from binding allolactose. This mutation does not affect DNA binding activity. If a plasmid copy of the lac operon containing the genotype Is, Oc, Z-, Y-, A+ is introduced into the E. coli strain described in Problem 15 above, which of the following statements would be TRUE?
lacA expression would be constitutive
lacY expression would be uninducible.
Transgenic plants can be generated by infecting plants with __________________ that carries the engineered transgene.
Which of the following statements best describes "Golden" rice?
Golden rice has been genetically engineered to produce increased levels of b-carotene
Addition of tryptophan to a minimal media liquid culture of an E. coli trpR mutant would have what effect on expression of the trp operon?
increase the rate of premature termination of trp operon transcription
The enzyme required to perform PCR is:
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Read the following description of a coastal arctic food web. Phytoplankton are the primary producers. Phytoplankton are eaten by zooplankton. Arctic cod, which eat zooplankton, are eaten by beluga whales, ringed seals, and walrus. Polar bears eat ringed seals. Draw the food web for this ecosystem. Categorize every organism into their trophic level. Explain why there are so few polar bears in arctic ecosystems.
Each of the following serves as an organ of gas exchange in frogs, toads, and many salamanders EXCEPT the a. skin. b. mouth cavity. c. lungs. d. nicitating membrane.
Does the rock cycle have a beginning and an end? In your answer, be sure to relate patterns within the rock cycle to surface and subsurface events.
Describe the relationships among temperature, heat, and thermal energy.