Chem Section Bank 2

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What quantity of NAG3 was required to reach the equivalence point in the titration?

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Terms in this set (10)

What quantity of NAG3 was required to reach the equivalence point in the titration?

Calm down when doing scientific notation conversions; also note that the equivalence point is when you have equal mols of titrant and titrant

KNAG3/Kapp = 1 + KNAG[NAG]

This is in slope intercept form, so to save time, you can see that KNAG is simply the slope of the line

An ITC experiment is conducted by injecting Compound 1 into a solution of Protein A giving large measured heats and a high affinity constant K1. A CITC experiment under identical conditions but in the presence of 10 mM Compound 2 gives the same heat curve and Kapp = K1. What change to the experimental conditions can result in measurable heat differences and Kapp < K1?

For a question like this, when stuck, refer back to the passage to see what the CITC is used for (competition for binding)

At pH 7, which of the following peptides will bind to an anion-exchange column and require the lowestconcentration of NaCl for elution?

Just count the amino acids one by one

For what mechanistic reason does G1 of lactase first act as a Brønsted acid during catalysis?

Look deeply at passage mxn and you'll see that the protonated oxygen is a good LG (water)

If [E]T was the concentration of lactase in the kinetics trials, what expression gives the concentration of lactase in the commercial preparation of this enzyme?

Didn't understand how to do dilution problems

What is the structure of the cytosine base after catalysis by Dnmt3a?

For rings with heteroatoms, start with the highest priority , then go around in a fashion assigning the heteroatoms lowest numbers

Which statement about the interaction between Dnmt3a and the DNA substrates is supported by the data in Figure 1?

50/50, go back to passage

Which protein has the highest electrophoretic mobility in SDS-PAGE under non-reducing conditions? (Note: There are no disulfide interactions unless stated in the table.)