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Terms in this set (22)
Eukaryotic pre-mRNA processing
5' cap, 3' poly-a-tail, splicing
function of HIV rev
exports 4kb spliced and complete unspliced HIV transcripts into the cytoplasm
HIV pol requires
slipping of the ribosome, causing a -1 frameshift
snRNPs are involved in
linkage that joins the base of the 5' cap to rest of mRNA
5' to 5'
function of aminoacyl-tRNA synthetase
attaches amino acids to 3' OH on tRNA
how many different polypeptide chains in a IgG antibody
two, two copies of heavy and two copies of light
If a heavy chain gene has 50 V segments, 25 D segments, and 6 J segments, how many different combinations can it make?
50 x 25 x 6 = 7500
functions of the 5' cap and poly-A-tail
protect mRNA from exonucleases and ribosomal recognition for translation initiation
how does translation terminate
release factor enters the ribosome when a stop codon is in the "A" site.
codon for the anticodon 5' -ACG- 3'
5' -CGU- 3'
how is the poly-A-tail added to mRNA
which part of an IgG antibody binds to antigen
tips of the "Y"
how many different antibodies are produced by a single B cell
in V-J recombination in antibody light chain genes, what happens to the excised DNA
becomes a circle and then gets lost
what enzyme modifies histones, leading to less tight binding to DNA
protein synthesis runs from
amino (NH2) to carboxy (COOH)
which ribosome binding site holds the tRNA attached to the growing peptide chain
what does reverse transcriptase use as a primer
function of HIV tat
why is HIV protease important for the virus?
it is needed to cut the polyproteins apart, releasing individual proteins.
what determines the position of translation initiation in prokaryotes
ribosome binds to Shine-Delgarno site and initiates at the next AUG
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