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34 terms

Enzymes

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catalase
substrate-H202
H202
reagent/indicator in catalase test
bubbles, s. epidermis
positive result of catalase test
oxidase
substrate-cytochrome
tetramethyl-p-phenylenediamine dihydrochloride
reagent/indicator in oxidase test
blue/purple, p. putida
positive result of oxidase test
amylase
substrate-starch
iodine
reagent/indicator in amylase test
zone of hydrolysis, B. subtilis
positive result of amylase
cellulase
substrate-cellulose
sheared/degraded/yellow, c. hutchinsonii
positive result of cellulase test
caseinase
substrate-casein
zone of hydrolysis, b. subtilis
positive result of caseinase test
gelatinase
substrate-gelatin
HCl
reagent/indicator in gelatinase
white ppt and zone of clearing, b. subtilis
positive result of gelatinase test
cysteine desulfhydrase
substrate-cysteine
ferric citrate
reagent/indicator in cysteine desulfhydrase test
black ppt, c. freundii
positive result of cysteine desulfhydrase test
trytophanase
substrate-tryptophan
kovacs reagent
reagent/indicator in trytophanase
red layer, E. coli
positive result of tryptophanase (indole test)
citrate-permease
substrate-citrate
bromthymol blue
reagent/indicator in citrate-permease
blue agar, E. aerogenes
positive result of citrate-permease test
nitrate reductase
substrate-nitrate
red color broth, E. coli
positive result of nitrate reductase
nitrite reductase
substrate-nitrite
nitrate reductase
enzyme tested for using the reagent/indicator alpha-napthol acetate and sulfanilic acid
gas bubbles, P. denitrificans
positive result of nitrite reductase
nitrogenase
substrate-dinitrogen (N2)
ability to grow, A. vinelandii
positive result of nitrogenase test
coagulase
substrate-fibrinogen
fibrin clots, S. aureus
positive result of coagulase test