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15 terms

Bio Lab Quiz 3 - Molecular Bio

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recombinant DNA technology
a process of introducing specific genes from one organism into another
transgenic organism
an organism with a gene from another species
restriction enzymes
enzymes that cut DNA molecules at specific sites and have become one of the basic tools of molecular biology.
polymerase chain reaction (PCR)
a process that enables scientists to make many copies (molecular clones) of the isolated DNA
DNA fingerprinting
analysis of fragments of DNA as a form of identification, used often in the criminal justice system
human genome
The scientific description of the complete set of DNA in the human organism, including gene locations
electrophoresis
a technique used to sort and separate large and small DNA fragments by size
gene mapping
technique that uses recombination frequencies to determine where the genes are located on the chromosomes.
restriction endonucleases
enzymes that help manipulate DNA molecules. They recognize a specific DNA sequence and cut the DNA at or near that site.
-nuclease
digestion of a nucleic acid (DNA)
Restricting
Cutting (digesting) requires energy in the form of ATP and involves a physical cleaving of chemical bonds
palindromic
sequence reads the same back and front. Most of the specific recognition sites where the cuts happen occur in-
base pair (bp)
a pair of complementary nitrogenous bases in a DNA molecule
plasmid
relatively small extrachromosomal and circular molecule of DNA found in bacteria and yeasts (pUC 19)
genomics
mapping the DNA fragments produced by restriction enzymes