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Diagnostic Microbiology (Microscopy and Staining)
Terms in this set (49)
Microscopy smears require at lest ____ organisms per mL
What are the things you have to take note of during microscopy?
Presence of inflammatory and non-inflammatory cells
Presence of these cells determine the adequacy of your sample
Non-inflammatory materials such as sq. epith cells?
Basis of gram staining
Bacterial cell wall
Gram Stain: secondary stain
Gram Stain: primary stain
Gram stain decolorizer
30% acetone alcohol
Gram stain mordant
Purpose of gram stain mordant?
What step in gram staining differentiates gram (+) from gram (-)?
What is the peptidoglycan layer?
Bacterial cell wall
Composition of Peptidoglycan layer
sugar backbone + peptide sidechains
Sugars seen in peptidoglycan layer
Monosaccharides: NAG and NAM
Which monosaccharide in the peptidoglycan layer is specific/unique to bacteria?
Purpose of peptidoglycan layer?
Protective layer + maintains shape of bacteria
Peptidoglycan layer of Gram (+) is ___ while gram (-) is ___
Teichoic acid is only present in Gram...
An outer membrane is only seen in Gram..
Components of outer membrane
Lipid A, O Antigen, LPS (lipopolysaccharides) aka endotoxins
All bacteria have cell walls except...
Only Gram (+) with LPS
LPS is only seen in the outer membranes and only gram (-) have outer membraenes...
Periplasmic space is seen in gram...
Teichoic acid's purpose is...
Organisms that can't be seen using Gram Staining Method
My My Stain Lang Cung otheR
This bacteria cannot be seen using gram stain because it contains too much lipid preventing the dye form penetrating the cell wall
This bacteria cannot be seen in gram stain because it is too thin
This bacteria cannot be seen using gram stain because it has poor uptake of red counterstain
These bacteria cannot be seen in gram stain because they are very small and both are intracellular
Chlamydia and Rickettsia
Alternative stain for mycobacteria
Acid Fast Stain
Alternative method to observe Spirochetes
Dark field Microscopy
Alternative method to observe mycoplasma spp
Alternative method for Legionella spp
Alternative method for Chlamyda
Alternative method for Rickettsia
Giemsa or Tissue staining
Enumerate the acid fast stains
Modified Acid Fast
Primary stain for Zhiel-neelsen
Counterstain for Zhiel-neelsen
This staining technique requires heating the specimen for uptake fo primary stain.
In zhiel-neelsen staining, organisms will appear color ___
Background olor of zhieel-neelsen
Also known as cold acid fast stain
Primayry stain of Auramine-Rhodamine
Counterstain of Auramine-Rhodamine
In auramine-rhodamine staining, organisms will appear color...
Background color of auramne-rhoadmine technique
Purpose of potassium permanganate in staining?
Inactivates unbound fluorescent dyes in auramine-rhodamine staining
Modified Acid Fast Stain uses___ + ___
Weak decolorizing agent + any of the 3 acid fast stains
Organisms that retain this stain are referred to as partially acid fast
Modified Acid Fast Stain
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