Upgrade to remove ads
Diagnostic Microbiology (Serology and Molecular tests)
Terms in this set (35)
Most useful when acute and convalescent sera are tested to show increase in Ab titers or time.
Serologic tests determine the presence of ___ to identify pathogenic agent.
antibodies in the serum
What cells produce anitbodies
Lowest dilution of the sample that retains detectable activity (concentration of Abs)
Antigens activate a distinct immune response with their...
anitgenic determinants or epitopes
Immunofluorescence uses what to detect Abs and antigens?
fluorescein-labled Abs aka fluorescein dye
When you want to test if px has bordatella pertussis, what serologic test can you use?
If you are trying to determine wether px has Legionella pneumophila infection, you use this serologic test
This type of immunofluoresence test uses fluorescein-dyed Abs which bind to antigens and emit light
This type of immunofluorescence test uses fluorescein-dyed antihuman-Abs which bind to Ab-Ag complexes and emit light.
This serologic test is best used to detect encapsulated microorganisms
Enumerate the encapsulated bacteria
Some Killers Have Pretty Nice Capsules
latex agglutination tests detect ___ of encapsulated microorganisms
carbohydrate (polysaccharide) antigens
What will be observed in a latex agglutination test if target Abs are present in the specimen and thy bind with Ag specific Abs?
Lattice formation = clumping
Meaning of ELISA
Enzyme Linked immunoabsorbant Assay
Specimen for ELISA
If you want to screen your px for HIV. what serologic test can you use?
Differentiate ELISA from RIA
ELISA uses enzyme-labled Abs or Antihuman-Abd
RIA uses radio-labled Abs or isotopes
ELISA uses spectophotometry (color) while RIA uses gamma counter (radioactivity)
If you want to test your px for EBV mononucleosis, what serologic test can you use?
If you are suspecting that your px may have syphilis and this is the first that he will undergo any firm of testing, what test can you use?
VDRL or RPR
Enumerate Non-treponemal tests for Syphillis
VDRL and RPR
Veneral Disease Research Labortory
Rapid plasma Reagin
Your px tested positive for syhpilis using RPR however, PE and Hx are both unremarkable. What is a possible explanation for this?
False positive due to cros reactivity secondary to
Specimen needed for VDRL
CSF or blood
This serologic test detects flocculation via microscopy
if your px tested positive for syphillis using RPR and his PE and Hx are congruent with the serologic test result, what is your next step?
Confirmatory test (treponemal tests)
Example of Treponemal test for Syphillis
Treponema Pallidum Enzyme Immunoassay (TPEIA)
This test obtains antigens from disrupted organisms and combine then with strips containing the specimen / Ab causing Abs to bind with protein bands (anitgen).
Western Blot Immunoassay
Westernblot : ___ :: Northern blot : ___ : Souther blot: ____
West: Proteins (Ab)
Adavntages of Molecular tests
Highly specific, highly sensitive, faster than culture
This test uses genotype to distinguish between related strains or microorganisms.
Example of Molecular test
Nucleic Acid Probes
Nucleic Acid Sequence Analysis
Nucleic acid amplification tests
This set is often in folders with...
Diagnostic Microbiology (Culture Media)
Diagnostic Microbiology (Specific Bacterial Cultur…
Diagnostic Microbiology (Microscopy and Staining)
Introduction to Microbiology
You might also like...
PCC BY 206 Lab Final
PMLS 1 MIDTERMS IMMUNO-SERO
Microbio Chapter 17: Infection diagnoses
Other sets by this creator
Systemic Opportunistic Infections
Intro to Mycology