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Lab exam 1-5
Terms in this set (27)
which parts of the micrscope are okay to hold when you carry the instrument
your microscope is considered to be a compound microscope. why?
it has ocular and objective lenses
the lenses that you look directly into are called the _____ lenses
the lenses that are attached to the revolving nosepiece are called objective lense. which objective lense has the highest magnifications
which part should I attempt to move to ensure the stage is as low as it will go?
which part of the microscope should you use to position the slide?
mechanical stage knobs
to find your specimen, all you need to do is?
lower the stage using the coarse focus
true or false: because the objective lenses are parfocal, it is not necessary for you to lower the stage before changing to a new objective
critical thinking: in addition to being in sharp focus, it was also important tohave the specimen centered in the field-of-view before changing objective lenses. this is because the diameter of the field-of-view will ___ when you increase magnification
why do we "sweep" the oil immersion lens all the way through the oil, then back again when changing to this lens?
to push any air bubbles trapped in the oil out of the way
what is the function of immersion oil?
it increases resolution
in general, as you increase magnification, you should also
increase lamp intensity
given that the ocular lenses have a magnification of 10x and the objective lenses have magnifications of 4x, 10x, 40x and 100x what is the total magnification when using the oil immersion objective
critical thinking: where should this glass slide be disposed of
a sharps container
after properly disposing of the slide, you clean the lenses of the microscope. what is the only thing that you should use to clean microscope lenses?
lens paper and lens cleaning solution
true or false: a single isolated colony should contain cells of only one species
what do we call the single cell or group of genetically-identical cells that was originally isolated on the spot where the colony formed?
when retrieving the cells from the plate, where should the lid be?
in your non-dominant hand, held above but still over the base
critical thinking: sometimes, the water falls to spread evenly across the slide when preparing a smear. what is the most likely explanation for this
the slide wasn't cleaned well enough
what is the purpose of this heat fixing step
to adhere the cells to the slide
how long should the crystal violet be applied
30 seconds to 1 minute
crystal violet is a basic dye. what does this mean
the dye has a positively charged chromophore
what difference would be observed in the outcome of this stain if you had used safranin rather than crystal violet
the cells would be pink instead of purple
imagine that after performing the stain, there were no cells visible anywhere on the slide. which of the following mistakes might explain this observation
the slide was not heat fixed long enough
In the differential staining, what is the "primary dye"
the dye that is applied first
in a differential staining procedure, what is a "mordant"
agent that enhances binding of a dye
in a differential staining procedure, what is the "counterstain"
dye that stains decolorized structures
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