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Bio 113 The molecular mechanism of transcription
Terms in this set (15)
What is the gene comprised of?
The first groups of the sequence is the promoter, the last few sequences is the termination signal. Neither of these code for a protein.
What does the transcription factor in prokaryotes do?
Is a protein (sigma). This binds to the RNA polymerase, guides it to the promoter and helps it by bind by recognizing two critical regions.
What does the sigma do?
Sigma recognizes and binds two critical areas of the promoter: -10box and -35box.
Where are these boxes located in relation to the coding region?
The -10box is up of the coming region.
the -35box is up of the -10box
What is the -10box?
The sequence is TATAAT.
Its ten nucleotides upstream from +1.
What is -35box?
The sequence is TTGACA.
It Is 35 nucleotides upstream from the +1
Where does RNA POLY begin transcription?
begins transcription at +1
How would a mutation in bacterial cell that deletes 3 nucleotides that are 10 nucleotides upstream from the transcription start site affect transcription?
Prevents transcription b/c mutation is in the critical area of the promoter
Transcription factors in eukaryotes?
They are more diverse
Some bind to DNA to guide RNA polymerase to the promoter.
Others bind to the DNA to remove chemical groups that are attached, making the DNA available to be bound.
making the mRNA in prokaryotes and eukaryotes
Once initiated, RNA polymerase continues transcription of the coding region and eventually transcribes the termination signal.
Stopping in prokaryotes and eukaryotes
Termination signal has lots of Cs and Gs.
The RNA folds back on itself, the Cs and Gs bind together, forming a hairpin.
The hairpin disrupts the interaction between RNA polymerase and the mRNA transcript, so the separate.
Eukaryotes- mRNA processing
in eukaryotes, the resulting mRNA is pre-mRNA and it needs to be processed before translation can occur.
(In prokaryotes, the resulting mRNA does not need to be processed)
What does a spliceosome do?
catalyzes the splicing reaction
composed of lots of small nuclear ribonucleoproteins (SnRNPs)=RNAs + proteins.
What do SnRNPs do?
Binds to intron boundaries and pinch them together until both ends are cut
Regulating which genes get expressed
Cells control which genes are transcribed at which times
this allows the cells to respond efficiently to changes in the environment and to have unique identity
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