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Exam 2 Quizes

Terms in this set (20)

Energy required to form product from substrate?
Activation Energy
Six Enzyme classes?
Oxidoreductase, Transferase, Hydrolase, Lyase, Isomerase, Ligase
Lysozyme catalyzes the hydrolytic cleavage of polysacchrides. what class of enzyme?
Hydrolase
What is Zymogen?
Inactive enzyme precursor.
Describe the mechanism of Chymotrypsins cleavage of a peptide bond.
See Chap. 8. and cry.
Which inhibition will shift the Km but not the Vmax of a reaction?
Competitive inhibition
Phosphofructokinase-1 is allosterically regulated by what two things? designate inhibitor or activator
PEP-inhibitor
ADP- activator
What is an example of Covalent modification?
Phosphorylation
Allosteric enzymes
Controlled using metabolic effectors.
Effectors bind noncovalently/reversibly to the enzymes they regulate.
Alternate between Taut and Relaxed forms.
Have multiple subunits.
Describe the difference between Essential ions and coenzymes.
Essential ions are metals loosely bound or part of enzyme active site. Coenzymes can be prosthetic groups or cofactors
Mammals need a source of coenzymes to survive, what essential compounds can these coenzymes be synthesized from?
Vitamins
Describe the difference between a cosubstrate and a prosthetic group.
Cosubstrate is a loosely bound coenzyme, a prosthetic group is usually a covalent modification.
NAD+ and NADP+ participate in what kind of cellular reactions.
Oxidation reduction reactions
an iron-sulfur complex is an example of what?
Metal ion of metalloenzymes
Name several cosubstrates that participate in oxidation-reduction reactions.
NADP+ and NADPH
NAD+ and NADH
ATP and ADP
RNA is transcribed
5' to 3'
Three main types of RNA
mRNA
tRNA
rRNA
In the absence of glucose
cAMP-CRP may dramatically activate transcription
Name two enzymes and their catalytic role in DNA synthesis or modification.
Helicase- unwinds DNA
Ligase- forms phosphodiester bonds
Endonuclease- cleaves phosphodiester bonds
Which of the following is not an example of RNA processing?
a)Removal of nucleotides from the primary transcript
b)Addition of nucleotides not encoded by the gene itself
c)Covalent modification of certain bases
d)Conformational changes due to CRP binding <----- Dat one
e)None of the above
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