Isolating and identifying a gene
Firstly, the nucleotide sequence of the G.O.I needs to be known. Then, the DNA containing the G.O.I is cut using restriction enzymes. From this, a radioactively labelled DNA Probe can be created that is complementary to the G.O.I. A solution containing the G.O.I is heated to separate the double strands, upon cooling, the DNA probe will bind to the G.O.I, thereby isolating and identifying the gene.