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Identification of Unknown Bacteria Lab (1)
Terms in this set (33)
What is the science of classifying microorganisms called?
What is the internationally accepted reference for bacterial classifications?
Bergey's Manual of Systematic Bacteria
What is the purpose of differential media?
Growth of bacteria while differentiating them from one another
What two medias primarily aid in the identification of bacteria?
Selective and Differential Media
What type of media allows for the growth of certain bacteria while preventing or retarding growth of other bacteria?
What type of media allows for growth of all bacteria while differentiating them from one another?
Selective and differential media used in the isolation of gram-positive cocci are used primarily for the identification of pathogenic______________ and _____________.
Streptococci and Staphylococci
What medias are commonly used to identify pathogenic streptococci and staphylococci?
Nutrient Agar and Mannitol Salt Agar
What is the concentration in Mannitol Salt Agar?
Certain bacteria can be identified on nutrient agar based on the ___________ they produce
Mannitol's salt concentration ______________ the growth of most bacteria other than ______________.
What color does staphylococci exhibit when in mannitol salt agar?
What color does other bacteria turn when grown in mannitol salt agar?
What two bacterium are indigenous to the skin?
Staphylococcus Epidermidis and Staphylococcus Aureus
What staphylococcus is usually responsible for infection?
What are a few side effects of infection of Staph. Aureus?
Lesions in the skin, infections of the heart, kidneys, and bladder
What types of enzymes are bacteria sometimes characterized by?
Exoenzymes and endoenzymes
Exoenzymes secrete enzymes...
outside of the bacteria and into the environment
What is catalase and what does it do?
Catalase is an enzyme that breaks down hydrogen peroxide into oxygen and water
Which bacterium is positive for catalase? How can you tell?
Staphylococcus; if you put hydrogen peroxide on staphylococcus bacteria, it will bubble and catalyze the hydrogen peroxide into water and oxygen
How does catalase assist a bacteria?
It helps the cell from becoming damaged by corrosive chemicals like hydrogen peroxide
What enzyme can determine between aerobic staph from the aerotolerant strep?
What two agars can be used for identifying gram-negative pathogenic bacteria?
MacKonkey, Eosin Methylene Blue, and Hektoen Enteric agar
What agar is both selective and differential?
How is MacKonkey agar both differential and selective?
The inhibitory action of crystal violet inhibits gram-positive cells while selecting out gram negative rods.
What two categories do we separate MacKonkey agar results into?
Coliform bacteria and Dysentery, typhoid, Paratyphoid bacilli
What bacteria in MacKonkey Agar are lactose fermenters?
What color do lactose fermenters turn? What is responsible for the color change?
Red, the low pH of lactic acid produced during lactose fermentation
What color does lactose turn when fermented on Eosin Methylene Blue agar agar?
What do you use Hektoen Enteric Agar for?
The identification of Enteric Bacteria and lactose fermenters
What are your two major types of Enteric bacteria?
Salmonella and Shigella
What color do lactose fermenters in HEA agar produce?
What color does hydrogen sulfide gas produce on HEA agar?
THIS SET IS OFTEN IN FOLDERS WITH...
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