Upgrade to remove ads
Only $35.99/year

MCB2010 Exam #1 Study Guide

Terms in this set (200)

E) They lack a plasma membrane.
Chapter 4
Which of the following statements is INCORRECT regarding prokaryotic cells?
A) Their DNA is not enclosed within a membrane.
B) They lack membrane-enclosed organelles.
C) They typically have a circular chromosome.
D) They reproduce by binary fission.
E) They lack a plasma membrane.
C) it protects the cell in a hypertonic environment.
Each of the following statements concerning the gram-positive cell wall is true EXCEPT
A) it maintains the shape of the cell.
B) it is sensitive to lysozyme.
C) it protects the cell in a hypertonic environment.
D) it contains teichoic acids.
E) it is sensitive to penicillin.
C) Water will move out of the cell.
Which of the following statements best describes what happens when a bacterial cell is placed in a solution containing 5% NaCl?
A) Sucrose will move into the cell from a higher to a lower concentration.
B) The cell will undergo osmotic lysis.
C) Water will move out of the cell.
D) Water will move into the cell.
E) No change will result; the solution is isotonic.
A) conjugation.
A gram-positive bacterium suddenly acquires resistance to the antibiotic methicillin. This trait most likely occurred due to acquisition of new genetic information through
A) conjugation.
B) binary fission.
C) meisosis.
D) transformation.
E) transduction.
C) active transport
By which of the following mechanisms can a cell transport a substance from a lower to a higher concentration?
A) simple diffusion
B) facilitated diffusion
C) active transport
D) extracellular enzymes
E) aquaporins
D) contains cholesterol
Which of the following is NOT a typical characteristic of most bacterial plasma membranes?
A) site of energy production
B) composed of a phospholipid bilayer
C) contains proteins
D) contains cholesterol
E) is selectively permeable
B) fungi
Which one of the following organisms has a cell wall?
A) protoplasts
B) fungi
C) L forms
D) mycoplasmas
E) animal cells
B) Endospores allow a cell to survive environmental changes by producing a dormant period with no growth.
Which of the following statements is TRUE?
A) Endospores are for reproduction.
B) Endospores allow a cell to survive environmental changes by producing a dormant period with no growth.
C) Endospores are easily stained in a Gram stain.
D) A cell produces one endospore and keeps growing.
E) A cell can produce many endospores.
D) centrosome — food storage
Which of the following pairs is mismatched?
A) endoplasmic reticulum — internal transport
B) Golgi complex — secretion
C) mitochondria — ATP production
D) centrosome — food storage
E) lysosome — digestive enzymes
B) mitochondrion
Which of the following organelles most closely resembles a prokaryotic cell?
A) nucleus
B) mitochondrion
C) Golgi complex
D) vacuole
E) cell wall
A) osmotic lysis.
Antibiotics that target cell wall synthesis ultimately cause bacterial cell death as a result of
A) osmotic lysis.
B) inhibition of molecular transport.
C) decreased synthesis of plasma membrane.
D) plasmolysis.
E) cell shrinkage.
A) lysozyme
Bacteria are a commonly used organism for studies of genetic material in the research laboratory. The nucleic acids must first be isolated from the cells for these studies. Which of the following would most likely be used to lyse gram-positive bacterial cells for nucleic acid isolation?
A) lysozyme
B) polymixins
C) alcohol
D) water
E) mycolic acid
D) They are sensitive to penicillin.
Which of the following statements about gram-negative cell walls is FALSE?
A) They protect the cell in a hypotonic environment.
B) They have an extra outer layer composed of lipoproteins, lipopolysaccharides, and phospholipids.
C) They are toxic to humans.
D) They are sensitive to penicillin.
E) Their Gram reaction is due to the outer membrane.
C) cilium
Which of the following structures is NOT found in some prokaryotic cells?
A) flagellum
B) axial filament
C) cilium
D) pilus
E) peritrichous flagella
E) binary fission.
Functions of the glycocalyx include all of the following EXCEPT
A) biofilm formation.
B) increased virulence.
C) source of nutrition.
D) protection against dehydration.
E) binary fission.
C) capsule
Which structure acts like an "invisibility cloak" and protects bacteria from being phagocytized?
A) slime layer
B) fimbriae
C) capsule
D) cell membrane
E) cell wall
C) ATP
Which of the following is NOT part of the passive transport process?
A) plasma membrane
B) transporter proteins
C) ATP
D) concentration gradient
E) aquaporins
C) lophotrichous flagella
Which of the following terms best describes the cell in Figure 4.1?
A) peritrichous flagella
B) amphitrichous flagella
C) lophotrichous flagella
D) monotrichous flagellum
E) axial filament
C) chromatophores.
In bacteria, photosynthetic pigments are found in
A) chloroplasts.
B) cytoplasm.
C) chromatophores.
D) mesosomes.
E) ribosomes.
D) requires transporter proteins.
The difference between simple diffusion and facilitated diffusion is that facilitated diffusion
A) moves materials from a higher to a lower concentration.
B) moves materials from a lower to a higher concentration.
C) requires ATP.
D) requires transporter proteins.
E) does not require ATP.
B) taxic movements of the cell in response to attractants or repellents.
The terms "run" and "tumble" are generally associated with
A) cell wall fluidity.
B) taxic movements of the cell in response to attractants or repellents.
C) clustering properties of certain rod-shaped bacteria.
D) cell membrane synthesis.
C) has a cell wall.
You have isolated a motile, gram-positive cell with no visible nucleus. You can safely assume that the cell
A) has 9 pairs + 2 flagella.
B) has a mitochondrion.
C) has a cell wall.
D) lives in an extreme environment.
E) has cilia.
D) pili are used for transfer of DNA and motility.
Fimbriae and pili differ in that
A) there are only one or two pili per cell.
B) pili are used for motility.
C) pili are used to transfer DNA.
D) pili are used for transfer of DNA and motility.
E) pili are used for attachment to surfaces.
B) b
In Figure 4.2, which diagram of a cell wall is a gram-negative cell wall?
A) a
B) b
C) both a and b
D) neither a nor b
E) The answer cannot be determined based on the information provided.
B) b
In Figure 4.2, which diagram of a cell wall possesses lipid A/endotoxin responsible for symptoms associated with infection?
A) a
B) b
C) both a and b
D) neither a nor b
E) The answer cannot be determined based on the information provided.
C) both a and b
In Figure 4.2, which diagram of a cell wall has a structure that protects against osmotic lysis?
A) a
B) b
C) both a and b
D) neither a nor b
E) The answer cannot be determined based on the information provided.
B) b
In Figure 4.2, which diagram of a cell wall is decolorized by alcohol?
A) a
B) b
C) both a and b
D) neither a nor b
E) The answer cannot be determined based on the information provided.
B) b
In Figure 4.2, which diagram of a cell wall is resistant to many antibiotics (e.g., penicillin)?
A) a
B) b
C) both a and b
D) neither a nor b
E) The answer cannot be determined based on the information provided.
B) b
In Figure 4.2, which diagram of a cell wall contains teichoic acids?
A) a
B) b
C) both a and b
D) neither a nor b
E) The answer cannot be determined based on the information provided.
A) a
In Figure 4.2, which diagram of a cell wall contains porins?
A) a
B) b
C) both a and b
D) neither a nor b
E) The answer cannot be determined based on the information provided.
C) the plasma membrane
Where are phospholipids most likely found in a prokaryotic cell?
A) flagella
B) around organelles
C) the plasma membrane
D) ribosomes
E) the plasma membrane and around organelles
E) the plasma membrane, around organelles, and surrounding flagella
Where are phospholipids most likely found in a eukaryotic cell?
A) surrounding flagella
B) around organelles
C) the plasma membrane
D) ribosomes
E) the plasma membrane, around organelles, and surrounding flagella
C) cell wall
Which of the following is NOT found or observed to occur in both mitochondria and prokaryotes?
A) circular chromosome
B) 70S ribosomes
C) cell wall
D) binary fission
E) ATP-generating mechanism
E) It involves movement of molecules down a concentration gradient and may require a transport protein.
Which of the following statements is correct about passive diffusion?
A) It requires an expenditure of energy by the cell.
B) It is a process in which molecules move from a region of higher concentration to a region of lower concentration (or down a concentration gradient).
C) It is a process in which molecules move from a region of lower concentration to one of higher concentration (or up a concentration gradient).
D) It may require a transport protein.
E) It involves movement of molecules down a concentration gradient and may require a transport protein.
B) through simple diffusion.
Oxygen crosses a plasma membrane
A) by osmosis.
B) through simple diffusion.
C) with the help of a nonspecific transporter.
D) through facilitated diffusion.
E) through porins.
D) plasmolyze.
In a hypertonic solution, a bacterial cell will typically
A) lyse.
B) burst.
C) stay the same.
D) plasmolyze.
E) osmolyze.
B) The cell will undergo osmotic lysis.
What will happen if a bacterial cell is pretreated with a lysozyme solution, then placed in distilled water?
A) The cell will plasmolyze.
B) The cell will undergo osmotic lysis.
C) Water will leave the cell.
D) Lysozyme will diffuse into the cell.
E) No change will result; the solution is isotonic.
C) Spirilla have an external flagella but spirochetes have axial filaments.
How do spirochetes and spirilla differ?
A) Spirochetes do not have a cell wall but spirilla do.
B) Spirilla are found in chains of cells whereas spirochetes exist as individual cells.
C) Spirilla have an external flagella but spirochetes have axial filaments.
D) Spirochetes have a rigid, corkscrew shape while spirilla are helical and more flexible.
E) Spirochetes and spirilla are basically the same organisms and the terms can be used interchangeably.
C) ribosomes - carbon storage
Which one of the following pairs is mismatched?
A) metachromatic granules - phosphate storage
B) lipid inclusions - energy reserve
C) ribosomes - carbon storage
D) sulfur granules - energy reserve
E) gas vacuoles - flotation
A) carboxysomes
Which of the following are NOT energy reserves?
A) carboxysomes
B) polysaccharide granules
C) lipid inclusions
D) ribosomes
E) metachromatic granules
D) cilia - pili
Which of the following is NOT a functionally analogous pair?
A) nucleus -nucleiod region
B) mitochondria - prokaryotic plasma membrane
C) chloroplasts - thylakoids
D) cilia - pili
E) 9+2 flagella - bacterial flagella
C) is circular in structure.
The DNA found in most bacterial cells
A) is surrounded by a nuclear membrane.
B) utilizes histones for chromosomal packaging.
C) is circular in structure.
D) is linear in structure.
E) is found in multiple copies.
A) TRUE
The cell walls of bacteria are responsible for the shape of the bacteria and the difference in the Gram stain reaction.
A) TRUE
B) FALSE
A) TRUE
Antibiotics that target the cell wall are an effective treatment against many pathogenic bacteria.
A) TRUE
B) FALSE
B) FALSE
Cells placed in a hypotonic solution tend to lose water due to osmotic pressure.
A) TRUE
B) FALSE
A) TRUE
Small, hydrophobic molecules pass through a cell membrane most easily by diffusion.
A) TRUE
B) FALSE
A) TRUE
Spheroplasts, protoplasts, and mycoplasms are bacterial cells without cell walls.
A) TRUE
B) FALSE
B) FALSE
Endospores are a reproductive structure.
A) TRUE
B) FALSE
A) TRUE
The internal structure of eukaryotic cilia and flagella are the same.
A) TRUE
B) FALSE
B) FALSE
Many enzymes in both prokaryotic and eukaryotic cells are compartmentalized within organelles.
A) TRUE
B) FALSE
B) FALSE
The number of organelles such as chloroplasts, mitochondria, and rough endoplasmic reticulum is the same in all eukaryotic cells.
A) TRUE
B) FALSE
A) TRUE
If you observe rod-shaped red cells after the Gram stain, you can assume their cell walls contain endotoxin.
A) TRUE
B) FALSE
C) coenzyme A
Chapter 5
Which of the following compounds is NOT an enzyme?
A) dehydrogenase
B) cellulase
C) coenzyme A
D) β-galactosidase
E) sucrase
C) NAD+
Which compound is being reduced in the reaction shown in Figure 5.1?
A) isocitric acid and α-ketoglutaric acid
B) α-ketoglutaric acid and NAD+
C) NAD+
D) NADH
E) NADH and isocitric acid
B) photoautotroph - CO2
Which organism is NOT correctly matched to its energy source?
A) photoheterotroph - light
B) photoautotroph - CO2
C) chemoautotroph - Fe2+
D) chemoheterotroph - glucose
E) chemoautotroph-NH3
C) It involves the reduction of an organic final electron acceptor.
Which of the following statements about anaerobic respiration is FALSE?
A) It yields lower amounts of ATP when compared to aerobic respiration.
B) The complete Kreb's cycle is utilized.
C) It involves the reduction of an organic final electron acceptor.
D) It generates ATP.
E) It requires cytochromes.
B) transamination
What type of reaction is in Figure 5.2?
A) decarboxylation
B) transamination
C) dehydrogenation
D) oxidation
E) reduction
E) It is converted into acetyl CoA.
What is the fate of pyruvic acid in an organism that uses aerobic respiration?
A) It is reduced to lactic acid.
B) It reacts with oxaloacetate to form citrate.
C) It is oxidized in the electron transport chain.
D) It is catabolized in glycolysis.
E) It is converted into acetyl CoA.
B) It would bind to b.
How would a noncompetitive inhibitor interfere with a reaction involving the enzyme shown in Figure 5.3?
A) It would bind to a.
B) It would bind to b.
C) It would bind to c.
D) It would bind to d.
E) The answer cannot be determined based on the information provided.
E) substrate-level phosphorylation
How is ATP generated in the reaction shown in Figure 5.4?
A) glycolysis
B) fermentation
C) photophosphorylation
D) oxidative phosphorylation
E) substrate-level phosphorylation
A) the Krebs cycle.
Fatty acids are oxidized in
A) the Krebs cycle.
B) the electron transport chain.
C) glycolysis.
D) the pentose phosphate pathway.
E) the Entner-Doudoroff pathway.
C) c
Which of the graphs in Figure 5.5 best illustrates the activity of an enzyme that is saturated with substrate?
A) a
B) b
C) c
D) d
E) e
B) A proton gradient allows hydrogen ions to flow back into the cells through transmembrane protein channels, releasing energy that is used to generate ATP.
Which of the following is the best definition of oxidative phosphorylation?
A) Electrons are passed through a series of carriers to O2.
B) A proton gradient allows hydrogen ions to flow back into the cells through transmembrane protein channels, releasing energy that is used to generate ATP.
C) ATP is directly transferred from a substrate to ADP.
D) Electrons are passed through a series of carriers to an organic compound.
D) The oxidation of intermediate metabolic compounds releases energy that is used to generate ATP.
Which of the following statements about substrate-level phosphorylation is FALSE?
A) It involves the direct transfer of a high-energy phosphate group from an intermediate metabolic compound to ADP.
B) No final electron acceptor is required.
C) It occurs in glycolysis.
D) The oxidation of intermediate metabolic compounds releases energy that is used to generate ATP.
E) It occurs to a lesser degree in the Krebs cycle than in glycolysis.
D) It requires CO2.
Which of the following statements about photophosphorylation is FALSE?
A) Light liberates an electron from chlorophyll.
B) The oxidation of carrier molecules releases energy.
C) Energy from oxidation reactions is used to generate ATP from ADP.
D) It requires CO2.
E) It occurs in photosynthesizing cells.
A) by glycolysis only
A strictly fermentative bacterium produces energy
A) by glycolysis only.
B) by aerobic respiration only.
C) by fermentation or aerobic respiration.
D) only in the absence of oxygen.
E) only in the presence of oxygen.
C) three ATPs.
The advantage of the pentose phosphate pathway is that it produces all of the following EXCEPT
A) precursors for nucleic acids.
B) precursors for the synthesis of glucose.
C) three ATPs.
D) NADPH.
E) precursors for the synthesis of amino acids.
D) beta oxidation
Which biochemical process is NOT used during glycolysis?
A) substrate-level phosphorylation
B) oxidation-reduction
C) carbohydrate catabolism
D) beta oxidation
E) enzymatic reactions
B) H2O.
In noncyclic photophosphorylation, O2 is released from
A) CO2.
B) H2O.
C) C6H12O6.
D) sunlight.
E) chlorophyll.
B) the partial oxidation of glucose with organic molecules serving as electron acceptors
Which of the following is the best definition of fermentation?
A) the partial reduction of glucose to pyruvic acid
B) the partial oxidation of glucose with organic molecules serving as electron acceptors
C) the complete catabolism of glucose to CO2 and H2O
D) the production of energy by oxidative-level phosphorylation
E) the production of energy by both substrate and oxidative phosphorylation
D) oxygen
Which of the following is NOT necessary for respiration?
A) cytochromes
B) flavoproteins
C) a source of electrons
D) oxygen
E) quinones
D) NADH
Which one of the following would you predict is an allosteric inhibitor of the Krebs cycle enzyme, α-ketoglutarate dehydrogenase?
A) citric acid
B) α-ketoglutaric acid
C) NAD+
D) NADH
E) ADP
D) H2S.
In green and purple bacteria, electrons to reduce CO2 can come from
A) CO2.
B) H2O.
C) C6H12O6.
D) H2S.
E) chlorophyll.
B) decrease.
Assume you are growing bacteria on a lipid medium that started at pH 7. The action of bacterial lipases should cause the pH of the medium to
A) increase.
B) decrease.
C) stay the same.
A) chemoautotroph
Which of the following uses CO2 for carbon and H2 for energy?
A) chemoautotroph
B) chemoheterotroph
C) photoautotroph
D) photoheterotroph
B) chemoheterotroph
Which of the following uses glucose for carbon and energy?
A) chemoautotroph
B) chemoheterotroph
C) photoautotroph
D) photoheterotroph
D) photoheterotroph
Which of the following has bacteriochlorophylls and uses alcohols for carbon?
A) chemoautotroph
B) chemoheterotroph
C) photoautotroph
D) photoheterotroph
C) photoautotroph.
Cyanobacteria are a type of
A) chemoautotroph.
B) chemoheterotroph.
C) photoautotroph.
D) photoheterotroph.
A) 2, 4, 5
Which of the following statements are TRUE?
1-Electron carriers are located at ribosomes.
2-ATP is a common intermediate between catabolic and anabolic pathways.
3-ATP is used for the long-term storage of energy and so is often found in storage granules.
4-Anaerobic organisms are capable of generating ATP via respiration.
5-ATP can be generated by the flow of protons across protein channels.
A) 2, 4, 5
B) 1, 3, 4
C) 2, 3, 5
D) 1, 2, 3
E) All of the statements are true.
C) heterolactic fermenters.
Microorganisms that catabolize sugars into ethanol and hydrogen gas would most likely be categorized as
A) aerobic respirers.
B) anaerobic respirers.
C) heterolactic fermenters.
D) homolactic fermenters.
E) alcohol fermenters.
D) Anabolic reactions are degradative.
Which of the following 81) Which of the following statements regarding metabolism is FALSE?
A) Heat may be released in both anabolic and catabolic reactions.
B) ATP is formed in catabolic reactions.
C) ADP is formed in anabolic reactions.
D) Anabolic reactions are degradative.
B) This process occurs anaerobically.
Which of the following is TRUE about this reaction?
NO3-+ 2H+ NO2- + H2O
Nitrate ion Nitrite ion

A) This process requires O2.
B) This process occurs anaerobically.
C) This process requires the entire electron transport system.
D) This process requires light.
E) This process requires O2 and the electron transport system.
D) ATP is generated.
Which of the following statements regarding the Entner-Doudoroff pathway is TRUE?
A) It involves glycolysis.
B) It involves the pentose phosphate pathway.
C) NADH is generated.
D) ATP is generated.
E) NADH and ATP are generated.
B) O2 is in the medium.
Assume you are working for a chemical company and are responsible for growing a yeast culture that produces ethanol. The yeasts are growing well on the maltose medium but are not producing alcohol. What is the most likely explanation?
A) The maltose is toxic.
B) O2 is in the medium.
C) Not enough protein is provided.
D) The temperature is too low.
E) The temperature is too high.
C) aerobic metabolism is more efficient than fermentation.
The rates of O2 and glucose consumption by a bacterial culture are shown in Figure 5.6. Assume a bacterial culture was grown in a glucose medium without O2. Then O2 was added at the time marked X. The data indicate that
A) these bacteria don't use O2.
B) these bacteria get more energy anaerobically.
C) aerobic metabolism is more efficient than fermentation.
D) these bacteria cannot grow anaerobically.
B) competitive inhibition.
An enzyme, citrate synthase, in the Krebs cycle is inhibited by ATP. This is an example of all of the following EXCEPT
A) allosteric inhibition.
B) competitive inhibition.
C) feedback inhibition.
D) noncompetitive inhibition.
C) pentose phosphate pathway
If a cell is starved for ATP, which of the following pathways would most likely be shut down?
A) Kreb's cycle
B) glycolysis
C) pentose phosphate pathway
D) Krebs cycle and glycolysis
D) One molecule of ATP is expended.
Which of the following statements regarding the glycolysis pathway is FALSE?
A) Two pyruvate molecules are generated.
B) Four ATP molecules are generated via substrate-level phosphorylation.
C) Two NADH molecules are generated.
D) One molecule of ATP is expended.
E) Two molecules of water are generated.
B) b
The graph at the left in Figure 5.7 shows the reaction rate for an enzyme at its optimum temperature. Which graph shows enzyme activity at a higher temperature?
A) a
B) b
C) c
D) d
C) using the peptides.
A bacterial culture grown in a glucose-peptide medium causes the pH to increase. The bacteria are most likely
A) fermenting the glucose.
B) oxidizing the glucose.
C) using the peptides.
D) not growing.
A) oxidation.
Gallionella bacteria can get energy from the reaction Fe2+ → Fe3+. This reaction is an example of
A) oxidation.
B) reduction.
C) fermentation.
D) photophosphorylation.
E) the Calvin-Benson cycle.
E) e
In Figure 5.8, where is ATP produced?
A) a
B) b
C) c
D) d
E) e
D) d
Refer to Figure 5.8. In aerobic respiration, where is water formed?
A) a
B) b
C) c
D) d
E) e
C) a plasma membrane.
In Figure 5.8, the structure labeled "1" is
A) NAD+.
B) ATP synthase.
C) a plasma membrane.
D) a cell wall.
E) cytoplasm.
B) protons.
In Figure 5.8, the path labeled "2" is the flow of
A) electrons.
B) protons.
C) energy.
D) water.
E) glucose.
A) a
What is the most acidic place in Figure 5.8?
A) a
B) b
C) c
D) d
E) e
B) M. tuberculosis produces urease.
A urease test is used to identify Mycobacterium tuberculosis because
A) urease is a sign of tuberculosis.
B) M. tuberculosis produces urease.
C) urea accumulates during tuberculosis.
D) some bacteria reduce nitrate ion.
E) M. bovis can cause tuberculosis.
A) an RNA molecule capable of catalysis.
Researchers are developing a ribozyme that cleaves the HIV genome. This pharmaceutical agent could be described as
A) an RNA molecule capable of catalysis.
B) a hydrolase.
C) a genetic transposable element.
D) a protease inhibitor.
E) a competitive inhibitor for reverse transcriptase.
B) 1, 2, 3
Which statements correspond to amphibolic pathways?
1. anabolic and catabolic reactions are joined through common intermediate
2. shared metabolic pathways
3. Feedback inhibition can help regulate rates of reactions
4. both types of reactions are necessary but do not occur simultaneously
A) 1 only
B) 1, 2, 3
C) 1, 2, 3, 4
D) 2, 4
E) 2, 3, 4
A) TRUE
Catabolic reactions are generally degradative and hydrolytic.
A) TRUE
B) FALSE
B) FALSE
The pentose phosphate pathway can be characterized as an anabolic pathway.
A) TRUE
B) FALSE
A) TRUE
In general, ATP is generated in catabolic pathways and expended in anabolic pathways.
A) TRUE
B) FALSE
A) TRUE
An apoenzyme that loses its coenzyme subunit will be non-functional.
A) TRUE
B) FALSE
B) FALSE
The use of enzymes is necessary to increase the activation energy requirements of a chemical reaction.
A) TRUE
B) FALSE
A) TRUE
Glycolysis is utilized by cells in both respiration and fermentation.
A) TRUE
B) FALSE
A) TRUE
Carbon fixation occurs during the light-independent phase of photosynthesis.
A) TRUE
B) FALSE
A) TRUE
Both respiration and photosynthesis require the use of an electron transport chain.
A) TRUE
B) FALSE
B) FALSE
Both respiration and photosynthesis use water molecules for the donation of hydrogen ions.
A) TRUE
B) FALSE
A) TRUE
Once an enzyme has converted substrates into products, the active site reverts back to its original form.
A) TRUE
B) FALSE
B) b
Chapter 6
Figure 6.1, which line best depicts a facultative anaerobe in the absence of O2?
A) a
B) b
C) c
C) c
In Figure 6.1,which line best depicts an obligate anaerobe in the presence of O2?
A) a
B) b
C) c
C) c
In Figure 6.1, which line shows the growth of an obligate aerobe incubated anaerobically?
A) a
B) b
C) c
A) a
In Figure 6.1, which line best illustrates the growth of a facultative anaerobe incubated aerobically?
A) a
B) b
C) c
B) b
In Figure 6.1, which line best depicts a psychrotroph incubated at 0°C?
A) a
B) b
C) c
D) DNA and proteins.
If cells are grown in media containing amino acids labeled with radioactive nitrogen (15N), most of the radioactivity will be found in the cells'
A) DNA.
B) proteins.
C) phospholipids.
D) DNA and proteins.
E) DNA and phospholipids.
E) phosphorus — used for production of carbohydrates.
Which of the following elements is NOT correctly matched with its cellular function?
A) nitrogen — needed for amino acid synthesis
B) phosphorus — incorporated into nucleic acids
C) sulfur — used for synthesis of thiamin and biotin
D) magnesium and potassium — required as cofactors for enzymes
E) phosphorus — used for production of carbohydrates.
B) capnophiles that grow best in carbon dioxide incubators.
Pathogenic bacteria isolated from the respiratory or intestinal tracts of humans are
A) strict aerobes that grow best in candle jars.
B) capnophiles that grow best in carbon dioxide incubators.
C) facultative anaerobes that require reducing media for growth.
D) strict aerobes that grow best in reducing media.
E) capnophiles that prefer highly oxygenated growth conditions.
A) BSL-1.
The biosafety level (BSL) for most introductory microbiology laboratories is
A) BSL-1.
B) BSL-2.
C) BSL-3.
D) BSL-4.
C) BSL-3.
The biosafety level (BSL) for a clinical microbiology laboratory working with potentially airborne pathogens, such as tuberculosis bacteria, is
A) BSL-1.
B) BSL-2.
C) BSL-3.
D) BSL-4.
D) 54,000 cells per milliliter
A sample of milk is tested for its bacterial content in a plate count assay. A one-milliliter sample of the milk is diluted in a 1:10 dilution series. One milliliter of the third dilution tube is plated in a pour plate. After incubation, the plate has 54 colonies, indicating that the original milk sample contained
A) 54 cells per milliliter.
B) 540 cells per milliliter.
C) 5,400 cells per milliliter.
D) 54,000 cells per milliliter.
E) 540,000 cells per milliliter.
A) buffers
The addition of which of the following to a culture medium will neutralize acids?
A) buffers
B) sugars
C) pH
D) heat
E) carbon
D) hypertonic environment.
Salts and sugars work to preserve foods by creating a
A) depletion of nutrients.
B) hypotonic environment.
C) lower osmotic pressure.
D) hypertonic environment.
E) lower pH.
A) does not use oxygen but tolerates it.
The term aerotolerant anaerobe refers to an organism that
A) does not use oxygen but tolerates it.
B) is killed by oxygen.
C) tolerates normal atmospheric nitrogen gas levels.
D) requires less oxygen than is present in air.
E) requires more oxygen than is present in air.
B) determines the number of viable cells
Which of the following is an advantage of the standard plate count?
A) can readily count cells that form aggregates
B) determines the number of viable cells
C) can be performed on very dilute samples, such as lake water
D) provides immediate results
E) can be used to count heat-sensitive bacteria
C) requires no incubation time
Which of the following is an advantage of the direct microscopic count?
A) can readily count organisms that are motile
B) can easily distinguish live from dead cells
C) requires no incubation time
D) sample volume is unknown
E) requires a large number of cells
D) binary fission.
Most bacteria reproduce by
A) aerial hyphae.
B) fragmentation.
C) mitosis.
D) binary fission.
E) budding.
D) 36 per milliliter
Thirty-six colonies grew in nutrient agar from 1.0 ml of undiluted sample in a standard plate count. How many cells were in the original sample?
A) 4 per milliliter
B) 9 per milliliter
C) 18 per milliliter
D) 36 per milliliter
E) 72 per milliliter
C) c
Figure 6.2 shows a typical bacterial growth curve with the y-axis indicating the log of the number of bacteria and the x-axis indicating time in culture. In the figure, which section (or sections) shows a growth phase where the number of cells dying equals the number of cells dividing?
A) a
B) b
C) c
D) d
E) a and c
B) b and d
Figure 6.2 shows a typical bacterial growth curve with the y-axis indicating the log of the number of bacteria and the x-axis indicating time in culture. In the figure, which sections of the graph illustrate a logarithmic change in cell numbers?
A) a and c
B) b and d
C) a and b
D) c and d
E) a and d
C) 7.
Most bacteria grow best at pH
A) 1.
B) 5.
C) 7.
D) 9.
E) 14.
B) 5.
Most fungi grow best at pH
A) 1.
B) 5.
C) 7.
D) 9.
E) 14.
D) selective and differential medium.
Consider a culture medium on which only gram-positive organisms such as Staphylococcus aureus colonies can grow due to an elevated NaCl level. A yellow halo surrounds the growth, indicating the bacterium fermented a sugar in the medium, decreasing the pH as a result and changing the color of a pH indicator chemical. This type of medium would be referred to as a(n)
A) selective medium.
B) differential medium.
C) enrichment culture.
D) selective and differential medium.
E) differential and enrichment culture.
B) complex medium.
A culture medium consisting of agar, peptone, and beef heart is a
A) chemically defined medium.
B) complex medium.
C) selective medium.
D) differential medium.
E) reducing medium.
B) thermophile — growth at 37°C
Which of the following pairs of microbe classification terms and optimal growth temperatures is mismatched?
A) psychrotroph — growth at 0°C
B) thermophile — growth at 37°C
C) mesophile — growth at 25°C
D) psychrophile — growth at 15°C
E) hyperthermophiles — growth at 85°C
B) log phase
During which growth phase will gram-positive bacteria be most susceptible to penicillin?
A) lag phase
B) log phase
C) death phase
D) stationary phase
E) The culture is equally susceptible during all phases.
B) the length of time needed for a cell to divide
Which of the following is the best definition of generation time?
A) the length of time needed for lag phase
B) the length of time needed for a cell to divide
C) the minimum rate of doubling
D) the duration of log phase
E) the time needed for nuclear division
D) metabolic activity
Which of the following is NOT a direct method to measure microbial growth?
A) direct microscopic count
B) standard plate count
C) filtration on a support membrane followed by incubation on medium
D) metabolic activity
E) most probable number (MPN)
B) facultative halophiles
Which group of microorganisms is most likely to spoil a freshwater trout preserved with salt?
A) psychrophiles
B) facultative halophiles
C) anaerobes
D) thermophiles
E) hyperthermophiles
B) vitamin B1
Which of the following is an organic growth factor?
A) glucose
B) vitamin B1
C) peptone
D) Mg+2
E) H2O
B) glucose consumption
Which of the following is an example of a metabolic activity that could be used to measure microbial growth?
A) standard plate count
B) glucose consumption
C) direct microscopic count
D) turbidity
E) most probable number (MPN)
D) 5
An experiment began with 4 cells and ended with 128 cells. How many generations did the
cells go through?
A) 64
B) 32
C) 6
D) 5
E) 4
C) 96
Three cells with generation times of 60 minutes are inoculated into a culture medium. How many cells are there after 5 hours?
A) 900
B) 180
C) 96
D) 32
E) 15
E) e
In Figure 6.3, which tube shows the expected growth pattern for a microaerophile?
A) a
B) b
C) c
D) d
E) e
B) b
In Figure 6.3, which tube shows the expected growth pattern for a facultative anaerobe?
A) a
B) b
C) c
D) d
E) e
B) a biofilm in the reprocessor
In one hospital, Pseudomonas aeruginosa serotype 10 infected the biliary tract of 10 percent of 1300 patients who underwent gastrointestinal endoscopic procedures. After each use, endoscopes were washed with an automatic reprocessor that flushed detergent and glutaraldehyde through the endoscopes, followed by a tap water rinse. P. aeruginosa serotype 10 was not isolated from the detergent, glutaraldehyde, or tap water. What was the source of the infections?
A) bacterial cell walls in the water
B) a biofilm in the reprocessor
C) contaminated disinfectant
D) fecal contamination of the bile ducts
E) None of the answers is correct.
C) the same number of cells in both.
Assume you inoculated 100 cells, with a generation time of 20 minutes, into 100 ml of nutrient broth. You then inoculated 100 cells of the same species into 200 ml of nutrient broth. After incubation for 4 hours, you can reasonably expect to have
A) more cells in the 100 ml.
B) more cells in the 200 ml.
C) the same number of cells in both.
D) The answer cannot be determined based on the information provided.
C) peptone and beef extract.
The source of nutrients in nutrient agar is
A) agar.
B) gelatin.
C) peptone and beef extract.
D) peptone and NaCl.
E) agar and NaCl.
D) superoxide dismutase
Which enzyme catalyzes the reaction: O2- + O2- + 2H+ → H2O2 + O2?
A) catalase
B) oxidase
C) peroxidase
D) superoxide dismutase
A) catalase
Which enzyme catalyzes the reaction: 2H2O2 → 2H2O + O2?
A) catalase
B) oxidase
C) peroxidase
D) superoxide dismutase
C) peroxidase
Which enzyme catalyzes the reaction: H2O2 + 2H+ → 2H2O?
A) catalase
B) oxidase
C) peroxidase
D) superoxide dismutase
C) facultative halophile.
The following data show growth of two bacteria on different media.
The data in Table 6.1 indicate that S. aureus is a(n)
A) mesophile.
B) facultative anaerobe.
C) facultative halophile.
D) aerobe.
E) halophile.
D) biofilms develop on catheters.
Patients with indwelling catheters (long-term tubes inserted into body orifices for drainage, such as through the urethra and into the urinary bladder) are susceptible to infections because
A) injected solutions are contaminated.
B) their immune systems are weakened.
C) infections can be transmitted from other people.
D) biofilms develop on catheters.
E) bacteria cause infections.
A) TRUE
An isolated colony on a streak plate contains millions (or even billions) of identical cells all arising from one initial cell.
A) TRUE
B) FALSE
A) TRUE
Bacterial growth refers to an increase in the numbers of cells in a bacterial culture.
A) TRUE
B) FALSE
B) FALSE
Pure cultures can easily be obtained on streak plates, even if the desired bacteria are present in very low concentrations in the initial culture broth.
A) TRUE
B) FALSE
A) TRUE
Agar is used as a solidifying agent in microbiological media since few bacteria can degrade it.
A) TRUE
B) FALSE
A) TRUE
Laboratory cultivation of obligate anaerobes requires reducing media or special growth chambers filled with inert gases.
A) TRUE
B) FALSE
B) FALSE
Most pathogenic bacteria are thermophiles.
A) TRUE
B) FALSE
B) FALSE
In performing a ten-fold dilutions series from a sample containing 10,000 bacteria per milliliter, the fourth tube in the dilution series will have 10 cells per milliliter.
A) TRUE
B) FALSE
A) TRUE
Turbidity is an indirect measurement of bacterial growth that can be measured using a spectrophotometer.
A) TRUE
B) FALSE
A) TRUE
Filtration methods, followed by growth of the bacteria trapped on the filters in growth media, are used to count bacteria present in very low concentrations, such as in lakes and streams.
A) TRUE
B) FALSE
A) TRUE
Nitrogen-fixing bacteria, such as cyanobacteria, can use atmospheric nitrogen (N2) for their nitrogen source.
A) TRUE
B) FALSE
C) membrane filtration
Chapter 7
Which of the following is the best method to sterilize heat-labile solutions?
A) dry heat
B) autoclave
C) membrane filtration
D) pasteurization
E) freezing
A) The cells in a population die at a constant rate.
Which of the following best describes the pattern of microbial death?
A) The cells in a population die at a constant rate.
B) All the cells in a culture die at once.
C) Not all of the cells in a culture are killed.
D) The pattern varies depending on the antimicrobial agent.
E) The pattern varies depending on the species.
C) ethylene oxide
Which of the following chemical agents is used for sterilization?
A) alcohol
B) phenolics
C) ethylene oxide
D) chlorine
E) soap
C) chlorhexidine
Which of the following substances is used for surgical hand scrubs?
A) phenol
B) chlorine bleach
C) chlorhexidine
D) soap
E) glutaraldehyde
A) bacteriostatic — kills vegetative bacterial cells
Which of the following pairs of terms is mismatched?
A) bacteriostatic — kills vegetative bacterial cells
B) germicide — kills microbes
C) virucide — inactivates viruses
D) sterilant — destroys all living microorganisms
E) fungicide — kills yeasts and molds
A) the formation of hypochlorous acid
The antimicrobial activity of chlorine is due to which of the following?
A) the formation of hypochlorous acid
B) the formation of hydrochloric acid
C) the formation of ozone
D) the formation of a hypochlorite ion
E) disruption of the plasma membrane
E) Alcohols effectively inactivate nonenveloped viruses by attacking lipids.
Which of the following regarding antimicrobial control agents is FALSE?
A) Contaminating organic debris such as blood or sputum decrease effectiveness.
B) Some agents kill by denaturing microbial cell proteins.
C) Some agents affect microbial cell membranes by dissolving lipids.
D) Silver is used for treating antibiotic-resistant bacteria.
E) Alcohols effectively inactivate nonenveloped viruses by attacking lipids.
B) pasteurization
Which of the following does NOT achieve sterilization?
A) dry heat
B) pasteurization
C) autoclave
D) supercritical fluids
E) ethylene oxide
D) It cannot be used with heat-labile materials.
Which of the following is a limitation of the autoclave?
A) It requires an excessively long time to achieve sterilization.
B) It cannot inactivate viruses.
C) It cannot kill endospores.
D) It cannot be used with heat-labile materials.
E) It cannot be used with glassware.
A) disinfectant.
An agent used to reduce the number of bacteria on a toilet would most accurately be called a(n)
A) disinfectant.
B) antiseptic.
C) aseptic.
D) fungicide.
E) virucide.
D) decreased thermal death time.
Application of heat to living cells can result in all of the following EXCEPT
A) breaking of hydrogen bonds.
B) alteration of membrane permeability.
C) denaturation of enzymes.
D) decreased thermal death time.
E) damage to nucleic acids.
C) bisphenols
Which of the following disinfectants acts by disrupting the plasma membrane?
A) soaps
B) aldehydes
C) bisphenols
D) halogens
E) heavy metals
B) glutaraldehyde.
Oxidizing agents include all of the following EXCEPT
A) chlorine.
B) glutaraldehyde.
C) hydrogen peroxide.
D) iodine.
E) ozone.
E) peracetic acid.
Disinfection of water is achieved by all of the following EXCEPT
A) ozone.
B) UV radiation.
C) chlorine.
D) copper sulfate.
E) peracetic acid.
A) alcohol.
All of the following substances are effective against nonenveloped viruses EXCEPT
A) alcohol.
B) chlorine.
C) ethylene oxide.
D) ozone.
E) None of the answers is correct; all of these are equally effective against nonenveloped viruses.
C) freezing
Which of the following methods is used to preserve food by slowing the metabolic processes of foodborne microbes?
A) lyophilization
B) nonionizing radiation
C) freezing
D) ionizing radiation
E) pasteurization
B) 70 percent
Which concentration of ethanol is the most effective bactericide?
A) 100 percent
B) 70 percent
C) 50 percent
D) 40 percent
E) 30 percent
E) None of the answers is correct; all of these factors may contribute to hospital-acquired infection.
All of the following factors contribute to hospital-acquired infections EXCEPT
A) some bacteria metabolize disinfectants.
B) gram-negative bacteria are often resistant to disinfectants.
C) invasive procedures can provide a portal of entry for bacteria.
D) bacteria may be present in commercial products such as mouthwash.
E) None of the answers is correct; all of these factors may contribute to hospital-acquired infection.
D) They are equivalent treatments.
Which of the following treatments is the most effective for controlling microbial growth?
A) 63°C for 30 minutes
B) 72°C for 15 seconds
C) 140°C for 4 seconds
D) They are equivalent treatments.
E) None of the answers is correct.
B) gamma radiation
Which of the following could be used to sterilize plastic Petri plates in a plastic wrapper?
A) autoclave
B) gamma radiation
C) microwaves
D) sunlight
E) ultraviolet radiation
E) A true antimicrobial control agent is equally effective against both bacteria and viruses.
Which of the following regarding antimicrobial control agents is FALSE?
A) Contaminating organic debris such as blood or sputum will decrease effectiveness.
B) Some agents kill by denaturing microbial cell proteins.
C) Some agents affect microbial cell membranes by dissolving lipids.
D) Some agents are utilized as both an antiseptic and a disinfectant.
E) A true antimicrobial control agent is equally effective against both bacteria and viruses.
B) 60 minutes
In Figure 7.1, what is the thermal death time?
A) 150°C
B) 60 minutes
C) 120 minutes
D) 100°C
E) The answer cannot be determined based on the information provided.
E) The answer cannot be determined based on the information provided
In Figure 7.1, the thermal death point for this culture is
A) 15 minutes.
B) 50°C.
C) 30 minutes.
D) 170°C.
E) The answer cannot be determined based on the information provided
B) 10 minutes.
In Figure 7.1, the decimal reduction time (D value) for the culture, which is defined as the time to reduce a population by one log, is approximately
A) 0 minutes.
B) 10 minutes.
C) 30 minutes.
D) 40 minutes.
E) 60 minutes.
E) heat, radiation, and some chemicals
Which of the following results in lethal damage to nucleic acids?
A) heat
B) radiation
C) certain chemicals
D) heat and radiation
E) heat, radiation, and some chemicals
B) alcohols — open wounds
Which of the following pairs is mismatched?
A) Ag — wound dressings
B) alcohols — open wounds
C) CuSO4 — algicide
D) H2O2 — open wounds
E) organic acids — food preservation
B) b
Which graph in Figure 7.2 best depicts the effect of placing the culture in an autoclave for 15 minutes at time x?
A) a
B) b
C) c
D) d
E) e
D) d
Which graph in Figure 7.2 best depicts the effect of placing the culture at 7°C at time x?
A) a
B) b
C) c
D) d
E) e
C) C
In Table 7.1, which compound was the most effective against Staphylococcus?
A) A
B) B
C) C
D) D
E) The answer cannot be determined based on the information provided.
E) The answer cannot be determined based on the information provided.
In Table 7.1, which compound was the most effective against E. coli?
A) A
B) B
C) C
D) D
E) The answer cannot be determined based on the information provided.
E) The answer cannot be determined based on the information provided.
In Table 7.1, which compound was bactericidal?
A) A
B) B
C) C
D) D
E) The answer cannot be determined based on the information provided.
B) sodium benzoate
In Table 7.2, which preservative is most effective?
A) potassium sorbate
B) sodium benzoate
C) potassium sorbate + sodium benzoate
D) no preservative
E) iodine mixed with a surfactant.
An iodophor is a(n)
A) phenol.
B) agent that reduces oxygen.
C) quaternary ammonium compound.
D) form of formaldehyde.
E) iodine mixed with a surfactant.
D) is a sterilizing agent.
Ethylene oxide
A) is a good antiseptic.
B) is not sporicidal.
C) requires high heat to be effective.
D) is a sterilizing agent.
E) is the active chemical in household bleach.
A) biguanides.
All of the following substances are used to preserve foods EXCEPT
A) biguanides.
B) nisin.
C) potassium sorbate.
D) sodium nitrite.
E) calcium propionate.
A) boiling.
All of the following are effective for destroying prions EXCEPT
A) boiling.
B) incineration.
C) NaOH + autoclaving at 134°C.
D) proteases.
E) None of the answers are correct; each of these will destroy prions.
E) ultraviolet radiation — desiccation
Which of the following pairs is mismatched?
A) ionizing radiation — hydroxyl radicals
B) ozone — takes electrons from substances
C) plasma sterilization — free radicals
D) supercritical fluids — CO2
E) ultraviolet radiation — desiccation
D) microwaves.
All of the following are methods of food preservation EXCEPT
A) desiccation.
B) high pressure.
C) ionizing radiation.
D) microwaves.
E) osmotic pressure.
Recommended textbook explanations

Fundamentals of Biochemistry

5th EditionCharlotte W. Pratt, Donald Voet, Judith G. Voet
980 explanations

Miller and Levine Biology

1st EditionJoseph S. Levine, Kenneth R. Miller
1,773 explanations

Fundamentals of Biochemistry: Life at the Molecular Level

5th EditionCharlotte W. Pratt, Donald Voet, Judith G. Voet
980 explanations

Modern Biology: Student Edition

1st EditionJanet L. Hopson, Postlethwait
2,490 explanations