Terms in this set (28)
This is the study of "genes in their entirety."
A map of the distribution of cloned genomic DNA from genomic clone libraries.
This term refers to the work undertaken by large teams of researchers who, through a concerted effort, clone and sequence the DNA of a particular organism.
A PCR technique that fills in small gaps by using the end of a cloned sequence as a primer to amplify into adjacent uncloned fragments.
A _______________ family is a group of evolutionarily related genes that arose through repeated evolution of an ancestral gene.
What is the purpose of an antibiotic resistance gene in a plasmid cloning vector?
To determine if the vector is present in host cell
A gene construct that indicates when transcription occurs because the protein is easily identified (often GUS or GFP).
What is a transgenic organism?
An organism that stably carries a foreign gene within is genome
The lungfish Protopterus aethiopicus has a genome 38 times larger than that of humans. Most of the DNA in this species is noncoding repetitive DNA. How could you create a library of clones that would let you compare just the genes in the lungfish to the genes in humans?
You could generate cDNA libraries and compare the transcribed regions of the genome
What is the purpose of the LacZ gene in a plasmid cloning vector?
To be able to distinguish those colonies with an insert using blue/white selection, white have an insert
The smallest number of clones that represents the entirety of the genome are called what?
minimum tiling path
Two genes that evolved from the same common ancestral gene, but are now found as homologs in different organisms are called _______________ .
in situ hybridization
A map of the order, overlap and orientation of physically isolated pieces of the genome.
What methods are used to produce mutations in a forward genetics approach?
UV light, EMS, Nitrosoguandandine, transposons
You have cut DNA from source A with restriction enzyme #1 and you have cut DNA from source B with restriction enzyme #2. Both of these restriction enzymes leave a 4 base single stranded overhang. You want to ligate these restricted fragments together. What must be true for this to be successful?
The RE must have complementary sticky ends so that they will anneal together
Another word for a "DNA chip" (microscopic spots of oligonucleotides bound to glass that can be fluorescently labelled to identify levels of expression).
List at least three different kinds of bacterial cloning vectors.
plasmid, phage, cosmid, BAC
List four uses of PCR
cell free cloning, screening for genetic disorders, forensics, paleobiology
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