Upgrade to remove ads
LAB: Bacterial Transformation
Terms in this set (11)
How many factors affect the transformation efficiency of E.Coli?
What is the first factor that affects the transformation efficiency of E.coli?
The foreign DNA must get through the Cell Wall
For DNA to pass through the cell wall, the cell wall must be __________________ but NOT destroyed. It uses an ice shock method.
The second factor that affects transformation efficiency of E.coli is that the DNA has to get through the ______________ membrane.
How does DNA pass through the plasma membrane?
(what process happens to allow DNA to pass through)
The plasma membrane is weakened by heat shock
The last factor that affects the transformation efficiency of E.coli is that the DNA must be incorporated into the __________ _____________.
The more ______________ injected, the more likely the bacteria will absorb it.
The type of ______________ can affect how easily/quickly bacteria takes it up.
The transformation ___________________ is 10^4 to 10^8 per ug of plasmid.
What is the transformation efficiency formula?
total # of colonies growing on agar plate/amount of DNA on LB/amp plate
An alternative to antibiotic resistance is the __________ gene, which confers bioluminescence.
This set is often in folders with...
Taxonomy of Biodiversity
Classification of Viruses
Evolution of Viruses
Other sets by this creator
Other Quizlet sets
Week 6 Assignment: Digital Imaging Characteristics