protein synthesis

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DNA is the molecule of heredity that contains instructions to build proteins. in 1958, francis crick described the central dogma of molecular biology explaining the process of gene expression. pick the correct term to fill in the blank spaces of the image.
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Terms in this set (34)
DNA is the molecule of heredity that contains instructions to build proteins. in 1958, francis crick described the central dogma of molecular biology explaining the process of gene expression. pick the correct term to fill in the blank spaces of the image.
transcription, translation
click view image to view the difference between the main characteristics of DNA and RNA. which statement is NOT correct?
RNA is less reactive
the mRNA strand is synthesized in the 5 to 3 direction. the circles in the image indicate the chemical group to which the next base is attached. which image has the circle in the correct position?
C (red circle on --OH of third carbon or bottom left)
DNA and mRNA synthesis proceeds in the five prime to three prime direction. what do five prime and three prime refer to?
the position of a carbon atom in the sugar backbone
protein synthesis is performed by an RNA protein complex called the ribosome. as you can see in the image, a ribosome is compromised of three sites called E, P & A. the site where tRNA adds its amino acids to the polypeptide chain is...
P
eukaryotic cells synthesize proteins in the ... which is shown as number ... in the image.
ribosomes, 2
what are the sequences of three nitrogen containing base of RNA that code for one amino acid called?
codon
the biosafety cabinet is important for physical containment. in this lab we will use a biosafety cabinet class too. who or what does a level two biosafety cabinet protect?
the personnel, the experiment, and the environment
the tRNA shown in the diagram will be able to form a base pair interaction with which mRNA?
5' - AGU
three types of RNA cooperate to perform translation. A; read codons and deliver the amino acids. B; carry the genetic information from DNA. C; catalyze the assembly of polypeptide chains. which type of RNA matches the A, B, and C descriptions, respectively?
tRNA, mRNA, rRNA
have a look at the simplified depiction of a DNA strand. which strand is the template strand?bottomwhich is the correct mRNA sequence resulting from the EPO gene transcription process?5' - AUU ACA AUG GGG GUG CAC GAA UGU CCU -3'what is the correct amino acid translated from the resulting mRNA?MGVHECPthe erythropoietin protein is 193 amino acids long, that means the number of nucleic acid in the mRNA molecule including the initiator in terminator codons are...582downstream means near the three prime end, so noticed that the first codon is not methionine. what is the correct amino acid sequence?TGDRamino acids are linked by covalent peptide bonds. the reaction of a peptide bond produces one water molecule. multiple amino acids are linked together by...peptide bondsa sequence of amino acids, or a polypeptide, grows from the N- terminus to the C- terminus. this polypeptide chain represents the primary structure of a protein. the linear sequence of amino acids within a protein is considered the ... structure of the protein.primarywhich level of protein structure is formed by the weak bonds between oxygen and hydrogen atoms within the polypeptide back down?secondarythe main to secondary structures of proteins are alpha helices and data sheets which are marked by ... and ... in the image.B and Abased on the mass spectrometer result, what is the mass difference between rhEPO expressed in CHO and E.Coli?12 kDaglycosylation sites are marked with a diagram of glycan. based on the mass spectrometry results, rhEPO is glycosylated in which expression system?CHObased on the mass spectrometry results, how many glycosylation sites exist in rhEPO-CHO?4bacterial cells often cannot produce recombinant proteins that are identical to Native mammalian proteins. the reason for this is that bacterial cells...lack the machinery for making post transitional modificationsrecombinant proteins produced by E. coli cells are not qlycosylated. this is because E.coli cells do not possess..golgi apparatusbased on the mass spectrometry results, the masses of our rhEPO expressed in CHO and E. coli are 30 kDa and 18 kDa, respectively. we also know that the EPO proteins expressed mammalian cells are glycosylated. therefore we can say that ... of rhEPO express in CHO are polysaccharides.0.4are mammalian expression systems better than the bacterial systems for the production of biologically active and stable erythropoietin?yes, erythropoietin is best expressed in mammalian cellsmass spectrometry identities molecules based on their...mass and chargeMALDI is an abbreviation for...matrix assisted laser desorption/ionizationmacromolecule ionization methods, such as MALDI MS, allow us to obtain a peptide mass fingerprint that can be used to...predict the identity of an unknown proteinX, Y, and Z refer to the parts of a mass spectrometer. X detects the number of different ions. Y is where ion production from the sample takes place. Z separates ions with different mass to charge ratio's. which diagram is the correct representation of X, Y, and Z?Y,Z,Xproteins samples are mixed with a matrix prior to analysis. what is the function of a matrix in MALDI MS analysis?ensures ionization of protein moleculesin mass spectrometry protein molecules are separated based on their mass to charge ratio. the data is recorded in a histogram for analysis. which of these histograms are not coherent with the mask spectrometry principal?II, III, IVwhich athlete is using rhEPO?CEPO can increase production of energy by increasing ... production.red blood cell