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what is the optimum temperature range for psychrotrophs?
what is the optimum temperature range for psychrophiles?
what is the optimum temperature range for mesophiles?
what is the optimum temperature range for thermophiles?
what would growth on the ocean floor be considered?
what would growth in a geyser be considered?
what would spoiled food in the refrigerator be considered?
what would cause disease in animals?
Will members of any of these groups (psychrophiles, mesophiles, thermophiles, psychrotrophs) grow in your freezer?
Will members of any of these groups (psychrophiles, mesophiles, thermophiles, psychrotrophs) survive in your freezer?
What is the term for a microbe that grows at 5C, 22C and 37C with optimum growth at 22C?
They denature and inactivate enzymes
How do high temperatures inhibit the growth of most bacteria?
diffusion of water across a semipermeable membrane
pressure due to osmosis created by a higher concentration of solute on one side of a semipermeable membrane
contains the same concentration of water and solutes as another solution separated from it by a semipermeable membrane
contains a higher concentration of water and a lower concentration of solutes than another solution separated from it by a semipermeable membrane
contains a lower concentration of water and a higher concentration of solutes than another solution separated from it by a semipermeable membrane
Isotonic- no change
Hypotonic- lysis without a cell membrane; microbes with a cell wall gain some water causing turgor pressure
Hypertonic- microbes lose water
explain what happens to microbes in each of the three types of environments
which of the three environments will inhibit bacterial growth?
microbe that require or can tolerate a high concentration of salt in its environment
microbe that can grow in a high salt or a high sugar environment
loss of water by microbes in a hypertonic environment
Which of the three cultures appears to be an osmophile?
yes, the 4% and 8% salt are hypertonic and will inhibit E. coli and Rhodotorula but not Staph. aureus which is a halophile. The 15% salt is very hypertonic and should inhibit the growth of all species
Was there any difference in the effect of the salt agar and sucrose agar on the growth of the microbes? If so, explain why there was a difference
microbes requiring atmospheric level (21%) of free O2 for growth
microbes requiring less than 21% free O2 for optimal growth
microbes that use free O2 when available but can also grow in its absence
microbes that cannot grow in the presence of free O2
microbes that grow equally well in presence or absence of free O2
In which of these groups (strict aerobe, microaerophile, facultative anaerobe, obligate anaerobe, aerotolerant anaerobe) can the Pasteur effect be observed?
Facultative Anaerobes and Aerotolerant Anaerobes
Which of the groups (strict aerobe, microaerophile, facultative anaerobe, obligate anaerobe, aerotolerant anaerobe) will grow BOTH on the anaerobic plate and on the aerobic plate?
Microaerophiles and Obligate Aerobes
Which in the group (strict aerobe, microaerophile, facultative anaerobe, obligate anaerobe, aerotolerant anaerobe) will grow ONLY on the aerobic plate?
Which of the groups (strict aerobe, microaerophile, facultative anaerobe, obligate anaerobe, aerotolerant anaerobe) will grow ONLY on the anaerobic plate?
Chemical reaction produces CO2 and H2 gas that combines with free O2 to form H2O
How is the oxygen removed in the anaerobic jar?
In the presence of oxygen, they produce toxic products like hydrogen peroxide that accumulates and kill them
How does oxygen prevent the growth of an obligate anaerobe?
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