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Enterotube Week - Module 8
Terms in this set (10)
The Enterotube II multiple test system was designed to allow the rapid identification of _______________________.
Bacteria in the family Enterobacteriaceae.
After inoculation of the Enterotube II, why are certain chambers punctured before incubation?
To create an aerobic environment
In the Enterotube II multiple test system, why are the last 4 chambers of the tube (glucose, ornithine decarboxylase, lysine decarboxylase. and H2S/Indole) not punctured when the other 8 chambers are?
To create an anaerobic environment
An enzyme that catalyzes the removal of a carboxyl group of an amino acid is called a _________.
The name of the enzyme that removes the amine group from phenylalanine is called ____________.
In the urea hydrolysis assay, phenol red indicator turns pink when the media becomes ____________________.
The image below is of a completed decarboxylation test for 3 different amino acids. What is the result for tube the tube labeled arginine (decarboxylase)? Can this bacterium decarboxylate arginine? The oil overlay is difficult to see in this picture.
Yes, ,the test is positive (purple)
The image below is of a completed decarboxylation test for 3 different amino acids. What is the result for tube the tube labeled lysine (decarboxylase)? Can this bacterium decarboxylate lysine? The oil overlay is difficult to see in this picture.
No, the test is negative (yellow)
The image below shows result of bacteria grown on phenylalanine deaminase media. After incubation, ferric chloride was added to the media. What is the result for tube b? Tube a is an uninoculated control.
Positive for deamination of phenylalanine (green growth)
The image below shows the results of a completed urease test for 2 bacteria after 24 hours of incubation. What is the result for tube B?
positive for rapid hydrolysis of urea (pink)
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