Upgrade to remove ads
Terms in this set (30)
What will happen if NLS or NES is ignored? (Unit 6)
you wouldn't be able to have certain proteins come in or out.
What does NLS and NES stand for? (Unit 6)
Nuclear localization sequence (NLS)- to enter
Nuclear export signals (NES)- to exit
Overview of protein import in the ER with ribosome (Unit 6)
1. Translation exposes signal sequence
2. SRP binds and arrests (pauses) translation
3. SRP docks the ribosome nascent chain complex at the ER to the translocon.
4. Hydrolysis occurs and the GDP is released then translocation begins.
5. Signal peptide is cleaved where the chain is folded.
6. Translation finishes, the channel closes and the ribosome dislocates.
Posttranslational translocation without ribosomes (Unit 6)
1. ATP is used to force the chain through the translocon.
2. BiP is bound to the ATP
3. Additional ATP binds to the chain to drag it through the translocon
Differences between using a ribosome and not with translocation (Unit 6)
Ribosome uses GTP for energy
Non-ribosomes is mostly in yeast and limited in higher eukaryotes
Difference between STA and SA (Unit 6)
-STA has no direction and needs a signal sequence
-SA acts as the anchor and signal sequence with the orientation towards the cytosol.
What would the sequence of the protein be? (Unit 6)
Change of membrane lipids (Unit 5)
Temp increase, fatty acids melt, fluid membrane increase, permeability increase, animals synthesize fatty acid chains to decrease fluidity.
Scientific process and pros/cons to the cell membrane models
Transverse diffusion (Unit 5)
Flipping or flip flopping of a lipid from one side to another of the membrane.
FRAP (Unit 5)
fluorescence recovery after photobleaching through exposing cells to high energy to figure out how long it will take to recover fluorescence to show migration of lipids.
ospin (Unit 5)
multichannel proteins that coat to help absorb light to make ATP with retinol
freeze fracturing (Unit 5)
Method of tissue preparation for transmission and scanning electron microscopy in which a tissue is frozen and a knife is then used to crack open the tissue; the fracture often occurs in the path of least resistance, within a membrane.
hydropathic analysis (Unit 5)
using a histograph to demonstrate how many times the polypeptide chain is passing through the membrane
secretion -cis (Unit 7)
means closer to the nucleus
trans- means (Unit 7)
away from the nucleus
anterograde means (Unit 7)
going towards the plasma (away from the nucleus)
retrograde means (Unit 7)
going away from the plasma (towards the nucleus)
why evolve endomembrane trafficking? (Unit 7)
can increase the material transportation being carried and the use of vesicles to transport hydrophilic material.
What is involved with the transport and secretion? (Unit 7)
ER, golgi and lysosomes
which organ in our body will be doing a lot of endomembrane? (Unit 7)
liver because it secretes proteins with the elaborate golgi
Steps of transportation- 1. Cargo selection (Unit 7)
any material being packaged into a vesicle can be highly selective and bulk transport.
Steps of transportation- 2. Budding (Unit 7)
binds to receptors, coat protein attach to vesicle to attach to adapter protein in order to stabilize, this includes COP I, II, and clathrin.
Steps of transportation- 3. Scission (Unit 7)
dynamin cuts the vesicle
Steps of transportation- 4. Uncoating (Unit 7)
uncoating of the coat and adapter proteins using Rab
Steps of transportation- 5. Transport (Unit 7)
sec21 will be attached to microtubules kinesin with use of energy to move the vesicle
Steps of transportation- 6. Tethering (Unit 7)
searching for the best area to dock for the vSNARE and tSNARE
Steps of transportation- 7. Docking (Unit 7)
vSNAREs are used to interact with tSNAREs
Steps of transportation- 8. Fusion (Unit 7)
Fusion of the membrane in order to release the cargo
Steps of transportation- 9. Disassembly (Unit 7)
proteins (NSF and SNAP) are used to disassemble while the vSNAREs and receptors are recycled
Sets found in the same folder
BIO 337 final exam part 1
Other sets by this creator
Biology Ch. 27-29 and 47
Biology 2- Exam 1
Other Quizlet sets
Western Civ. The Prince
sensation and perception exam 2
To measure the amount of dissolved oxygen in a sam…