Mallory Trichrome staining is a good stain to use for what?distinguishing intracellular vs. extracellular componentsWhen using Mallory Trichrome staining, what color do different substances stain?-Collagen fibers: intense blue
-Mucus and ground substance: varying shades of blue
-Cytoplasm and neuroglia: red
-Elastic fibrils, RBC, and nucleoli: pink or yellowWhen studying slides, you will come across artifacts. Common artifacts include-tissue cracking (due in part to poor fixation)
-postmortem degeneration
-knife marks
-shrinkage
-precipitates
-dust and folds in the specimenplasma membrane functionprotect the cell from the surrounding environment as it regulates what comes in and outlipid bilayer-polar head is hydrophilic
-fatty acid tail is hydrophobicnucleushouses the DNArough ER-with ribosomes makes proteins
-contains membrane bound ribosomessmooth ER-metabolizes lipids, synthesizes cholesterol and steroid-based hormones, detoxify drugs and certain pesticides
-does not contain membrane-bound ribosomesribosome functionproduce proteinsGolgi complexpackages proteins, lipids, enzymes for export by vesiclesmitochondria-power plant of the cell
-aerobic respirationMitochondria are numerous in metabolically active tissues. List a few sites where you would expect to find them in high numbersfat cells
muscle cellsLysosomes-clean up mechanism in cells
-low pH environment
-contain enzymes that break down almost all biological materialsWhat are the elements of the cytoskeleton?-microtubule
-intermediate filaments
-actinActin filamentscell structure, motility, migration
-wound healing
-muscle contraction
-cytokinesisIntermediate filaments-most stable and permanent cytoskeletal structure
-neurofilaments in nerve cells and keratin filaments in epitheliumMicrotubules-motor proteins move "cargo" along microtubular "railroad tracks"
-chromosome separation during cell division
-determine cell shape and distribution of organelles
