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Lec 25 Bone Marrow and Cytochemistry

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Romanowsky stain

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Terms in this set (51)

Romanowsky stain

● Consists of Methylene Blue and Eosin
● Oxidized methylene blue forms azures or basic compounds that stain acidic components of cell (nucleus)
● Eosin, acidic dye, binds to basic cellular structures like cytoplasm and Hgb
● ex: Wright, Wright-Giemsa, May-Grunwald, Lishman, Jenner

How do cell components stain?

○ PMN granules, slightly basic, stain weakly with azures - lilac
○ Eos granules, basic, stain with eosin - red/orange
○ Basophils - acidic, stain w/ azure, black/dark purple

made from concentrated WBC
○ do when diff WBC <2500
○ perform diff on buffy coat
○ perform WBC estimate, RBC morphology, and platelet estimate on regular smear

○ proximal portion of long bones in adults (location of medullary hematopoiesis)
○ at birth, all bones contain hematopoietic marrow but fat replaces marrow age 5-7

Normal vs Fatty BM (bone marrow)

○ Normal BM (bone marrow) is rusty red, thick fluid consistency, with various amounts of fat and spicules (gray white marrow fragments)
○ Need spicules present to be able to analyze
○ Fatty BM is yellow and haematopoietically inactive