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Lab (thin-layer Chromatography)
Terms in this set (16)
Thin Layer Chromatography (TLC)
A type of chromatography that uses silica gel or alumina on a card as the medium for the stationary phase
Distance moved by spot/
Distance moved by solvent
(distance traveled by solute)/(distance traveled by solvent)
How do you calculate the Rf value of a substance?
a technique in which separation is achieved by equilibration of solute between two phases
a technique in which solute equilibrates between the mobile phase and adsorption sites on the stationary phase
adsorption of the solute onto the matrix
What is the main principle in play during adsorption chromatography?
the solubility of the solute into the solvent
what is the main principle in play in partition chromatography?
If a molecule is no very soluble in the solvent will it travel farther or less far in TLC?
If a molecule is very soluble in the solvent will it travel farther or less far in TLC?
rubbing alcohol, much less polar than water
solute A would travel farther as the partition chromatography effect would be in play more than the adsorption chromatography
If solute A is nonpolar, what effect would adding more isopropanol to the solvent have on the distance it travels?
by comparing Rf values with known values
How does TLC help identify compounds?
TLC relies on the relative affinity of the solute with which phase of the process?
polar adsorbent and slightly acidic, this gives powerful ability to absorb basic contents
describe silica gel in TLC
detection of compound in mixture, establish to compounds of different origin are the same, determine number of compound present, choosing the appropriate solvent for column chromatography, monitoring reactions
what are the main uses of TLC?
the ratio of the initial and final solution volumes
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