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Lab Quiz 5: Bioenergetics
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The loss of the "blue" methylene blue color accompanied which of the following?
oxidation of succinic acid
A 24 year old male works for UPS. He loads heavy parcels on the truck 5 days a week. When he returns home after work, he runs 5 miles every day before resistance training for 30 minutes to build his muscles. What is his level of Voluntary Muscle Activity (VMA)?
100% X BMR
Which of the following is not related to BMR?
lifestyle
What would happen to an individual's weight if they ate 2500 Cal/day, and used 2500 Cal/day?
no change in weight
Which of the choices below describes an individual that eats 2500 Calories per day, sits at work and only watches TV at home? Their energy expenditure is 2000 Calories per day.
They are sedentary and will gain weight
Which of the following can be used for energy production?
sugar and amino acids
How many Calories can be produced from 1 g of protein or carbohydrate?
4 cal
A candidate antiviral drug to treat COVID-19 infections could be a competitive inhibitor of certain viral enzymes. Would this type of drug completely inhibit viral replication by itself?
No
At low substrate concentrations, as the substrate concentration increases, enzyme activity:
Increases
Once a high substrate concentration is achieved, the enzyme becomes saturated. This is because:
the active sites of all available enzyme molecules are filled
Which of the following can be ignored when determining your Daily Caloric Intake?
the 2 L of water you drank per day
Which of the following does not contribute to basal metabolism?
contraction of the rectus femoris
To test tube #2 of the hamburger experiment we added hamburger, water and methylene blue. Why did we see a reaction when succinic acid was not added?
there was already succinic acid in the hamburger
The conversion of succinic acid to fumaric acid is part of which of the following processes?
the Krebs Cycle
What types of chemical reactions are detected by methylene blue?
oxidation and reduction
What role did malonic acid play in the conversion of succinic acid to fumaric acid?
competitive inhibitor
Which of the following caused methylene blue to lose its "blue" color during the "hamburger" experiment?
the gain of 2 H+, reduction
What effect does decreasing temperature or diluting an enzyme have on enzyme activity? Do either of these treatments harm the enzyme?
both decrease, no harm
What is the role of caffeine in energy drinks?
stimulant
What would happen to an individual's weight if they ate 2500 Cal/day, and used 2000 Cal/day
increase
Which test tube was the negative control in the hamburger experiment?
the test tube with no hamburger
A healthy diet should include:
1/2 a plate of vegetables per meal
What is the effect of a competitive inhibitor on the saturation level of an enzyme?
decrease
At the end of the "hamburger" experiment, methylene blue loses its "blue" color from the bottom of the test tube first. A persistent blue ring remains at the surface of the mixture because this surface is in contact with:
oxygen
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